Purified anti-FIS Antibody

Pricing & Availability
Clone
Poly28613 (See other available formats)
Other Names
Mitochondrial fission 1 protein, FIS1, Tetratricopeptide repeat protein 11, TPR repeat protein 11, TTC11, FIS1 Homolog, Fission, Mitochondrial 1, hFIS1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28613_Purified_FIS_Antibody_1_051319.png
Western blot of purified anti-FIS antibody (Poly28613). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28613_Purified_FIS_Antibody_1_051319.png
    Western blot of purified anti-FIS antibody (Poly28613). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28613_Purified_FIS_Antibody_2_051319.png
    IHC staining of purified anti-FIS antibody (Poly28613) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28613_Purified_FIS_Antibody_3_051319.png
    IHC staining of purified anti-FIS antibody (Poly28613) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28613_Purified_FIS_Antibody_4_051319.png
    IHC staining of purified anti-FIS antibody (Poly28613) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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861301 25 µg £64
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Description

Mitochondria are dynamic organelles that vary in copy number, size, and position in any give cell type, often reflecting the energy requirements of the cell. Fusion and Fission is a well conserved and complex process for mitochondria morphology homeostasis, dysfunction of which is linked to many disorders. Human FIS1 protein, a homolog of yeast FIS1p, was identified as a mitochondria outer membrane protein. Overexpression of FIS1 leads to mitochondrial fragmentation and aggregation in mammalian cells caused by excessive fission events. Aberrant mitochondrial fragmentation leads to  cytochrome C release and induction of apoptosis. Depletion of Fis1 induces mitochondrial fusion and elongation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant FIS1 (Met1-Asp121) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human FIS1 is a 152 amino acid protein with a molecular mass of 17 kD.
Distribution

Tissue distribution: Ubiquitously expressed
Cellular distribution: Peroxisome and mitochondria

Function
FIS1 is involved in the fragmentation of the mitochondrial network and its perinuclear patterning.
Interaction
Interacts with DNM1L/DLP1, MARCH5, MIEF1, PEX11A, PEX11B, PEX11G.
Antigen References
  1. Stojanovski D, et al. 2004. J Cell Sci. 117(Pt 7):1201-10.
  2. James DI, et al. 2003. J Biol Chem. 278(38):36373-9.
Gene ID
51024 View all products for this Gene ID
UniProt
View information about FIS on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/13/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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