Purified anti-Fibrinogen β Antibody

Pricing & Availability
Clone
Poly28636 (See other available formats)
Other Names
Fibrinogen Beta Chain, Fibrinogen, B Beta Polypeptide, Beta-Fibrinogen, Epididymis Secretory Sperm Binding Protein Li 78p, HEL-S-78p
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28636_PURE_Fibrinogen-B_Antibody_1_102219
Western blot of purified anti-Fibrinogen β antibody (Poly28636). Lane 1: Molecular weight marker; Lane 2: 10 µg of human liver lysate; Lane 3: 10 µg of mouse liver lysate; Lane 4: 10 µg of rat liver lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28636_PURE_Fibrinogen-B_Antibody_1_102219
    Western blot of purified anti-Fibrinogen β antibody (Poly28636). Lane 1: Molecular weight marker; Lane 2: 10 µg of human liver lysate; Lane 3: 10 µg of mouse liver lysate; Lane 4: 10 µg of rat liver lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28636_PURE_Fibrinogen-B_Antibody_2_102219
    IHC staining of purified anti-Fibrinogen β antibody (Poly28636) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28636_PURE_Fibrinogen-B_Antibody_3_102219
    IHC staining of purified anti-Fibrinogen β antibody (Poly28636) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28636_PURE_Fibrinogen-B_Antibody_4_102219
    IHC staining of purified anti-Fibrinogen β antibody (Poly28636) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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863601 25 µg £64
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Description

Fibrinogen β is the β subunit of Fibrinogen, which is a blood-borne glycoprotein. Fibrinogen β, together with Fibrinogen α and γ subunits, forms fibrin matrix, which functions as the primary component of blood clots during vascular injury. Fibrinogen β is also involved in cell adhesion and chemotactic signaling pathway. Mutations in Fibrinogen β is associated with diseases, including Dysfibrinogenemia, Congenital.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Fibrinogen β expressed in E.coli.
Formulation
0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Fibrinogen β is a 491 amino acid protein with an apparent molecular mass of ~ 56 kD.
Distribution

Tissue Distribution: Blood, immune system, nervous system
Cellular Distribution: Extracellular, membrane

Function
Fibrinogen β is involved in cell adhesion and chemotactic signaling pathway.
Interaction
Fibrinogen α, Fibrinogen γ, thrombin
Cell Type
B cells, Mesenchymal Stem Cells, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neurodegeneration, Neuroinflammation
Molecular Family
Adhesion Molecules, APP/β-Amyloid, Innate Immune Signaling
Antigen References
  1. Bychkova AV, et al. 2017. Dokl Biochem Biophys. 474(1):173-177.
  2. Ahn HJ, et al. 2017. Curr Opin Hematol. 24(5):427-431.
  3. Luo H, et al. 2019. J Stroke Cerebrovasc Dis. 28(2):243-250.
Gene ID
2244 View all products for this Gene ID 110135 View all products for this Gene ID 24366 View all products for this Gene ID
UniProt
View information about Fibrinogen beta on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/22/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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