- 10F4H9D12 (See other available formats)
- Regulatory Status
- Other Names
- F-BOX/LLR-repeat protein 5, FBL5
- Mouse IgG2a
- Ave. Rating
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- Product Citations
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In metazoans, iron homeostasis is mediated at the systemic and cellular levels by the RNA-binding iron regulatory proteins IRP1 and IRP2.Under conditions where cellular iron is limiting, IRP1 and IRP2 stabilize the mRNA of genes involved in iron acquisition and mobilization and translationally repress genes involved in iron storage and export. Under iron replete conditions, both IRPs are inactivated: IRP1 acquires an Fe-S cluster that inhibits RNA-binding activity, while IRP2 is targeted for proteasomal-mediated degradation by the E3 ubiquitin ligase FBXL5. Under iron-replete conditions Fe-S cluster biogenesis is disrupted, FBXL5 will also target IRP1 for degradation.
FBXL5, or F-box and leucine-rich repeat protein 5 (FBXL5), is the E3 ubiquitin ligase component of the SCFFBXL5 complex. The complex contains SKP1, Cullin-1, RBX1, and FBXL5. When cytosolic iron is replete, FBXL5 recruits IRP2 to the SCF complex and promotes its ubiquitination and subsequent degradation. FBXL5 contains a hemerythrin-like domain that senses oxygen and iron availability. This sensing mechanism is linked to the stability of the FBXL5 protein itself: when cytosolic iron is available, iron binds to the hemerythrin domain and promotes the stability of the FBXL5 protein. When iron is deficient, the FBXL5 protein lacks the iron ligand, resulting in its degradation and activation of the IRP-mediated iron starvation response.
Studies have also shown that FBXL5 can ubiquitinate and target the SNAIL1 transcription factor and disruption of this process promotes epithelialto mesenchymal transition.
- Antibody Type
- Host Species
- Recombinant human FBXL5
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml (1:500 - 1:2000). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone may require prolonged exposure times to visualize proteins by western blot. We strongly recommend loading at least 30 µg of protein to facilitate detection.
(PubMed link indicates BioLegend citation)
- Moroishi T, et al. 2014. J Biol Chem. 289: 16430. (WB)
- Chollangi S, et al. 2012. J Biol Chem. 287: 23710. (WB)
AB_2749876 (BioLegend Cat. No. 672603)
AB_2749875 (BioLegend Cat. No. 672604)
- FBXL5 is a 691 amino acid protein with a predicted molecular weight of 78.6 kD. The protein contains an N-terminal hemerythrin like domain, followed by a F-box domain and seven leurcine-rich repeats.
Cytosol and nucleus; ubiquitous expression
- Systemic and cellular iron homeostasis
- IRP2, IRP1, SKP1, Cullin-1, RBX1, HERC2
- Iron and oxygen
- Biology Area
- Cell Biology, Ubiquitin/Protein Degradation
- Antigen References
- Johnson NB, et al. 2017. J. Biol. Chem. 292: 15976
- Vinas-Castells R, et al. 2014. Nucleic Acids Res. 42: 1079
- Moroishi T, et al. 2014. J Biol Chem. 289: 16430.
- Ruiz JC, et al. 2013. J Biol Chem 288:552.
- Chollangi S, et al. 2012. J Biol Chem. 287: 23710.
- Moroishi T, et al. 2011. Cell Metab. 14:339
- Salahudeen AA, et al. 2009. Science. 326: 722
- Vashisht AA, et al. 2009. Science. 326: 718
- Gene ID
- 2634 View all products for this Gene ID
- View information about FBXL5 on UniProt.org
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