Purified anti-EML4 Antibody

Pricing & Availability
Clone
W18139A (See other available formats)
Other Names
EML4, Echinoderm microtubule-associated protein-like 4
Isotype
Rat IgG2b, κ
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Product Citations
publications
W18139A_PURE_EML4_Antibody_1_091019.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-EML4 Antibody, clone W18139A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Cell lines were loaded in order of increasing predicted EML4 expression (source: Human Protein Atlas).
  • W18139A_PURE_EML4_Antibody_1_091019.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-EML4 Antibody, clone W18139A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Cell lines were loaded in order of increasing predicted EML4 expression (source: Human Protein Atlas).
  • W18139A_PURE_EML4_Antibody_2_091019.png
    HepG2 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold Methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of either Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602, panel A) or Purified anti-EML4 Antibody, clone W18139A (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
670801 25 µg £57
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670802 100 µg £144
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Description

Echinoderm microtubule-associated protein-like 4 or EML4 plays a central role in the modification of microtubule structural dynamics and morphology. During mitosis, EML4 expression is upregulated and is associated with spindle formation. In lung cancer cells, a translocation mutation is associated with the EML4 gene, where parts of the gene fuses with the anaplastic lymphoma receptor tyrosine kinase (ALK) gene and generates an EML4-ALK fusion protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human EML4 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg/mL. For immunocytochemistry, a concentration of 1.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has not been tested for IP.

RRID
AB_2814524 (BioLegend Cat. No. 670801)
AB_2814525 (BioLegend Cat. No. 670802)

Antigen Details

Structure
EML4 is a 981 amino acid protein with a predicted molecular weight of 109 kD
Distribution

Cytoskeleton/Ubiquitously expressed

Function
Microtubule formation
Antigen References
  1. Yoshida A, et al. 2011. J Thorac Oncol. 10:1677-86.
  2. Soda M, et al. 2007. Nature. 448:561.
  3. Pollmann M, et al. 2006. Exp Cell Res. (17):3241-51.

 

Gene ID
27436 View all products for this Gene ID
UniProt
View information about EML4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/10/2019

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