Purified anti-Elastin Antibody

Pricing & Availability
Clone
BL28631 (See other available formats)
Other Names
Elastin, Tropoelastin, ADCL1, SVAS, WBS
Isotype
Mouse IgG1, κ
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Product Citations
publications
BL28631_PURE_Elastin_Antibody_1_102319
Western blot of purified anti-Elastin antibody (clone BL28631). Lane 1: Molecular weight marker; Lane 2: 20 µg of human A431 cell lysate. The blot was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28631_PURE_Elastin_Antibody_1_102319
    Western blot of purified anti-Elastin antibody (clone BL28631). Lane 1: Molecular weight marker; Lane 2: 20 µg of human A431 cell lysate. The blot was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28631_PURE_Elastin_Antibody_2_102319
    IHC staining of purified anti-Elastin antibody (clone BL28631) on formalin-fixed paraffin-embedded human lung tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
863101 25 µg £60
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Description

Elastin is a protein that composes elastic fibers in extracellular matrix to confer elasticity of organs including skin, lung, and colon. Elastokines, which are fragments degraded from elastin, stimulate proliferation and migration of skin fibroblasts and monocytes, through ERK and integrin signaling pathways. Elastins are involved in cancer progression and diseases including supravalvular aortic stenosis and cutis laxa.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Elastin expressed in E.coli.
Formulation
0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage, aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Elastin is a 786 amino acid protein with an apparent molecular mass of ~68 kD.
Distribution

Tissue Distribution: Heart, kidney, lung, skin
Cellular Distribution: Extracelluar, membrane

Function
Elastin is involved in skin fibroblast migration and proliferation, and cancer progression.
Interaction
MFAP2, FBN1, FBLN2, FBLN5 and MFAP4
Cell Type
Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cardiovascular Biology, Cell Biology, Signal Transduction
Molecular Family
Adhesion Molecules
Antigen References
  1. Weihermann AC, et al. 2017. Int J Cosmet Sci. 39(3):241-247.
  2. Wahart A, et al. 2019. FEBS J. 286(15):2980-2993.
Gene ID
2006 View all products for this Gene ID
UniProt
View information about Elastin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/23/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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