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Purified anti-eIF3F Antibody

Pricing & Availability
Clone
W19170B (See other available formats)
Regulatory Status
RUO
Other Names
Eukaryotic Translation Initiation Factor 3 Subunit F, Deubiquitinating Enzyme EIF3f, EIF3-Epsilon, EIF3 P47, MRT67
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19170B_PURE_eIF3F_Antibody_1_082820.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-eIF3F antibody (clone W19170B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19170B_PURE_eIF3F_Antibody_1_082820.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-eIF3F antibody (clone W19170B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19170B_PURE_eIF3F_Antibody_2_082820.png
    Total cell lysates (15 µg total protein) from HEK-293 cells treated with non-targeting control siRNA (siCtrl) or siRNA directed against eIF3F (sieIF3F) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-eIF3F antibody (clone W19170B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
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939401 25 µg £94
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939402 100 µg £230
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Description

The eukaryotic translation initiation factor 3 (eIF3) is an 800 kD oligomeric complex comprised of 13 different subunits and plays an essential role in protein translation. The eIF3 complex directly links the 43S pre-initiation complex to eIF4F-bound mRNA. The subunit eIF3F has been shown to negatively regulate both cap-dependent and IRES-dependent translation and also interacts with mTOR and S6K1, and therefore also regulates mTORC1 and mTORC2 signaling. EIF3F mRNA is downregulated in multiple types of cancer. Overexpression of eIF3F protein leads to hypertrophy by altering protein synthesis via the mTORC1 pathway. Under energy starvation conditions, eIF3F is ubiquitinated by the MAFbx/atrogen-1 complex and targeted for proteasomal degradation, leading to muscle atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human eIF3F protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in poor eIF3F staining.

This clone failed to immunoprecipitate eIF3F from HeLa lysates.

RRID
AB_2876767 (BioLegend Cat. No. 939401)
AB_2876767 (BioLegend Cat. No. 939402)

Antigen Details

Structure
eIF3F is a 357 amino acid protein with a predicted molecular weight of 38 kD.
Distribution

Ubiquitously expressed/Cytoplasm

Function
Protein Translation
Biology Area
Cell Biology, Protein Synthesis
Antigen References
  1. Docquier A, et al. 2019. J Physiol. 597:3107.
  2. Sanchez AM, et al. 2013. Int J Biochem Cell Biol. 45:2158.
  3. Wen F, et al. 2012. PLoS One. 7:e34194.
  4. Csibi A, et al. 2010. PLoS One. 5:e8994.
  5. Doldan A, et al. 2008. Mol Carcinog.47:235.
Gene ID
8665 View all products for this Gene ID
UniProt
View information about eIF3F on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/27/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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