Purified anti-EAAT2 Antibody

Pricing & Availability
Clone
A18128E (See other available formats)
Other Names
GLT1, SLC1A2, Excitotoxic Amino Acid Transporter 2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18128E_PURE_EAAT2_Antibody_082019
Western blot of purified anti-EAAT2 antibody (clone A18128E). Lane 1: Molecular weight marker; Lane 2: 20 ug of human brain lysate; Lane 3: 20 ug of mouse brain lysate; Lane 4: 20 ug of rat brain lysate. The blot was incubated with 2 ug/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18128E_PURE_EAAT2_Antibody_082019
    Western blot of purified anti-EAAT2 antibody (clone A18128E). Lane 1: Molecular weight marker; Lane 2: 20 ug of human brain lysate; Lane 3: 20 ug of mouse brain lysate; Lane 4: 20 ug of rat brain lysate. The blot was incubated with 2 ug/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
869201 25 µg £60
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869202 100 µg £158
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Description

EAAT2 is the major membrane protein that clears extracellular glutamate from the synaptic area to prevent potential neuronal excitotoxicity from excessive activation of glutamate receptors. EAAT2 is predominantly expressed in the central nervous system on astrocytes and is frequently used as a marker for these cells. Mutations that affect EAAT2 function are clinically associated with amyotrophic lateral sclerosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant mouse SLC1A2 (EAAT2) protein (a.a. 143 - 238) with GST Tag
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814631 (BioLegend Cat. No. 869201)
AB_2814632 (BioLegend Cat. No. 869202)

Antigen Details

Structure
EAAT2 is a 574 amino acid protein with predicted and observed molecular mass of ~ 62 kD.
Distribution

Tissue Distribution: Brain

Cellular Distribution: Plasma membrane

Function
EAAT2 is a sodium-dependent amino acid transporter that mediates the uptake of L-Glutamate and L- or D- aspartate.
Interaction
EAAT2 forms homotrimer and interacts with JUB.
Cell Type
Astrocytes
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Pregnolato S, et al. 2019. Front Physiol. 10:417
  2. Parkin GM, et al. 2018. World J Psychiatry. 10.5498/wjp.v8.i2.51 
  3. Rosenblum LT, Trotti D. 2017. Adv. Neurobiol. 16:117-136. 
Gene ID
6506 View all products for this Gene ID 20511 View all products for this Gene ID 29482 View all products for this Gene ID
UniProt
View information about EAAT2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/20/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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