Purified anti-Dynactin 1 (N-Terminus) Antibody

Pricing & Availability
Clone
3D5-C6-D5 (See other available formats)
Other Names
DCTN1, Dynactin Subunit 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
3D5-C6-D5_PURE_Dynactin-1_Antibody_070919.png
Western blot of purified anti-Dynactin 1 (N-Terminus) antibody (clone 3D5-C6-D5). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of human kidney lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Note that the antibody detects both the brain specific isoform at~135 kD and the ubiquitously expressed isoform at ~150kD.
  • 3D5-C6-D5_PURE_Dynactin-1_Antibody_070919.png
    Western blot of purified anti-Dynactin 1 (N-Terminus) antibody (clone 3D5-C6-D5). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of human kidney lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Note that the antibody detects both the brain specific isoform at~135 kD and the ubiquitously expressed isoform at ~150kD.
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867601 25 µg £60
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867602 100 µg £158
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Description

Dynactin 1 is the largest subunit of the macromolecular complex dynactin, which is essential for dynein-mediated retrograde transport of vesicles and organelles. Dynactin 1 is clinically identified as a causative gene for Perry syndrome (PS), which is a neurodegenerative disease characterized by autosomal dominant inheritance, parkinsonism, and central hypoventilation. Mutations in dynactin 1 are also associated with neurodegenerative diseases, such as amyotrophic lateral sclerosis and frontotemporal dementia.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant Dynactin 1 protein fragments expressed in E.coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Dynactin 1 is a 1278 amino acid protein with two reported isoforms. A brain-specific isoform with predicted and observed molecular mass of ~135 kD, and a ubiquitously expressed isoform with predicted and observed molecular mass of ~150 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytoskeleton, cytosol, and nucleus

Function
Dynactin 1 recruits dynein to microtubules and facilitates retrograde transport of vesicles and organelles.
Interaction
Dynactin 1 forms homodimer and interacts with dynein.
Biology Area
Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family
Adaptor Proteins, Carrier Proteins, Cytoskeletal Proteins, Neurotransmitter Receptor Trafficking/Endocytosis, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Konno T, et al. 2017. Parkinsonism Relat Disord. 41:14.
  2. Houlden H, et al. 2012. Acta Neuropath. 124(3):325.
  3. Tanaka F, et al. 2011. Rinsho Shinkeigaku. 51(11):1189.
Gene ID
1639 View all products for this Gene ID
UniProt
View information about Dynactin 1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/09/2019

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