Purified anti-Dopamine Receptor D1 Antibody

Pricing & Availability
Clone
A17050E (See other available formats)
Other Names
DRD1, Dopamine Receptor D1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17050E_Pure_Dopamine-Receptor-D1_Antibody_1_110818
Western blot of purified anti-Dopamine Receptor D1 antibody (clone A17050E). Lane 1: Molecular weight marker; Lane 2: 40 µg of mouse brain lysate; Lane 3: 40 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_1_110818
    Western blot of purified anti-Dopamine Receptor D1 antibody (clone A17050E). Lane 1: Molecular weight marker; Lane 2: 40 µg of mouse brain lysate; Lane 3: 40 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_2_110818
    Western blot of purified anti-Dopamine Receptor D1 antibody (clone A17050E). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate membrane fraction; Lane 3: 40 µg of human brain total lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_3_110818
    IHC staining of purified anti-Dopamine Receptor D1 antibody (clone A17050E) on formalin-fixed paraffin-embedded human Substantia Nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_4_110818
    IHC staining of purified anti-Dopamine Receptor D1 antibody (clone A17050E) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_5_110818
    IHC staining of purified anti-Dopamine Receptor D1 antibody (clone A17050E) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
856001 25 µg £60
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856002 100 µg £158
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Description

The D1 subtype of the dopamine receptor (DRD1) is a Gs/a-protein coupled receptor mainly expressed in the caudate putamen of the central nervous system. Upon binding to its ligand, DRD1 indirectly activates cyclic AMP-dependent protein kinase and regulates neuronal development. Two transcript variants, DRD1a and DRD1b exist in vivo. DRD1 has been discovered to modulate DRD2-mediated signaling pathway. DRD1 and DRD2 also form heterodimers via C-terminal regions, which has been reported to be involved in phospholipase C and CaMKII activation, and BDNF production.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human DRD1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783448 (BioLegend Cat. No. 856001)
AB_2783449 (BioLegend Cat. No. 856002)

Antigen Details

Structure
DRD1 is a 446 amino acid protein with an apparent molecular mass of ~ 49 kD.
Distribution

Tissue Distribution: Mainly expressed in the midbrain.
Cellular Distribution: Plasma membrane.

Function
DRD1 is involved in dopamine related signaling and neuronal development.
Interaction
Interacts with DRD2, DNAJC14, and DRD1IP.
Ligand Receptor
Receptor of Dopamine
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References
  1. Amare AT, et al. 2017. EPMA J. 8(3):211
  2. Arango-Lievano M, et al. 2015. Med Sci (Paris). 31(5):546
  3. Serretti A, et al. 2007. J Alzheimers Dis. 12(1):73
Gene ID
1812 View all products for this Gene ID 13488 View all products for this Gene ID
UniProt
View information about Dopamine Receptor D1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/08/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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