Purified anti-DEPTOR Antibody

Pricing & Availability
Clone
W17128A (See other available formats)
Other Names
DEPTOR, DEPDC6
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17128A_Purified_DEPTOR_Antibody_1_122718
Western blot analysis of 15 µg cell lysates from Daudi, HeLa (Human), Raw264.7 (Mouse), UMR106 (Rat) and CHO (Hamster) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL purified anti-DEPTOR antibody, clone W17128A, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 607904, 1:10000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • W17128A_Purified_DEPTOR_Antibody_1_122718
    Western blot analysis of 15 µg cell lysates from Daudi, HeLa (Human), Raw264.7 (Mouse), UMR106 (Rat) and CHO (Hamster) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL purified anti-DEPTOR antibody, clone W17128A, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 607904, 1:10000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • W17128A_Purified_DEPTOR_Antibody_2_122718
    Western blot analysis of 15 µg cell lysates from Daudi cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 5, 2, 0.5 and 0.1 µg/mL purified anti-DEPTOR antibody, clone W17128A, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 664804, 1:25000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
Cat # Size Price Quantity Avail. Save
610201 25 µg £57
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610202 100 µg £144
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Description

DEPTOR/DEPDC6, found only in vertebrates, plays an important role in multiple cellular processes such as cell growth, autophagy, apoptosis, ER stress and adipogenesis. It inbibits mTORC1 and mTORC2 kinase activity through direct interaction with mTOR in these complexes. DEPTOR also associates with chromatin and may regulate transcription, and can act as either an oncogene or oncosuppressor depending on cell- or tissue-specific context.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Hamster
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human DEPTOR Protein (1-219 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783359 (BioLegend Cat. No. 610201)
AB_2783360 (BioLegend Cat. No. 610202)

Antigen Details

Distribution

Cytoplasm/ Nucleus, A little bit more in Cytoplasm

Gene ID
64798 View all products for this Gene ID
UniProt
View information about DEPTOR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/27/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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