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Purified anti-DDX3X Antibody

Pricing & Availability
Clone
15D1B11 (See other available formats)
Regulatory Status
RUO
Other Names
ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal (DBX), DDX3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
15D1B11_DDX3X_Purified_Antibody_WB_012014
Total cell lysates (15 µg total protein) from Daudi (reduced expression control), HeLa, SH-SY5Y, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-DDX3X antibody, clone 15D1B11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 15D1B11_DDX3X_Purified_Antibody_WB_012014
    Total cell lysates (15 µg total protein) from Daudi (reduced expression control), HeLa, SH-SY5Y, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-DDX3X antibody, clone 15D1B11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 15D1B11_Pure_DDX3X_Antibody_3_092518
    Immunoprecipitation of DDX3X from Hela cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-DDX3X antibody (clone 15D1B11, lane 4). Western blot was performed using anti-DDX3X antibody (clone 15D1B11). The astrostar indicates immunoglobulin heavy chain.
  • Z_15D1B11_DDX3X_Purified_Antibody_IF_011515
    Hela cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti-DDX3X antibody (15D1B11) and followed by DyLight™ 594 goat anti-mouse IgG (red) for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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658602 100 µg £238
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Description

DDX3X belongs to the DEAD (Asp-Glu-Ala-Asp)-box protein family of RNA helicases, involved in the alteration of RNA secondary structure required for transcription, mRNA processing, and translation initiation. DDX3X is an essential host factor during virus infection. DDX3X serves as the cellular target of Hepatitis C virus (HCV) core protein, and also facilitates nuclear export of immature HIV RNA. The RNA helicase activity of DDX3X is required for the replication of both HCV and HIV. DDX3X is implied to act as a viral RNA sensor, mediating antiviral innate immune signaling. DDX3 participates in IκB kinase ε (IKKε) and TANK-binding kinase 1 (TBK1) dependent IRF3 activation, leading to the production of Type I interferon. DDX3X was also proposed to have antiapoptotic functions and participate in tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human DDX3X recombinant protein (1-226aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Gordon DE, et al. 2020. Molecular Cell. 78(2):197-209. PubMed
RRID
AB_2562957 (BioLegend Cat. No. 658602)

Antigen Details

Structure
662 amino acids, predicted molecular weight of 73 kD. Contains a ATP-dependent helicase domain.
Distribution

Nucleus, Cytoplasm

Function
DDX3X is an ATP-dependent RNA helicase, involving in mRNA transcription, maturation, export, and translation.
Interaction
Interacts with DDX5, DDX58, IKKε, TBK1, XPO1, TDRD3, PABPC1, NXF1, EIF3C, MAVS, and NCAPH.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Protein Synthesis
Antigen References

1. Lai MC, et al. 2013. PLoS One. 8:e68665.
2. Bol GM, et al. 2013. PLoS One. 8:e63548.
3. Soto-Rifo R, et al. 2012. EMBO J. 31:3745.
4. Gu L, et al. 2013. Mol. Cell Biol. 33:2004.
5. Wang H, et al. 2009. J. Virol. 83:5815.
6. Lee CS, et al. 2008. Nucleic Acids Res. 36:4708.

Gene ID
1654 View all products for this Gene ID
UniProt
View information about DDX3X on UniProt.org

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For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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