Purified anti-CSTB Antibody

Pricing & Availability
Clone
BL28583 (See other available formats)
Other Names
Cystatin B, CSTB, Stefin B, CST6, STFB
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
BL28583_PURE_CSTB_Antibody_1_082119
Western blot of purified anti-CSTB antibody (BL28583). Lane 1: Molecular weight marker; Lane 2: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28583_PURE_CSTB_Antibody_1_082119
    Western blot of purified anti-CSTB antibody (BL28583). Lane 1: Molecular weight marker; Lane 2: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
858301 25 µg £60
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Description

Cystatin B (CSTB) is a type 1 cystatin belonging to the cystatin superfamily. There are three cystatin subfamilies including type 1 cystatins, type 2 cystatins, and kininogens. Cystatins are thought to have a protective role against the proteases that leak from the lysosomes by inhibiting their cysteine proteinase activity. CSTB can covalently form dimers, tetramers, or oligomers. CSTB oligomers bind to aggregated amyloid-beta (Aβ) and reduce Aβ fibril formation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant rat CSTB (amino acid sequence Met2~Phe98)
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814580 (BioLegend Cat. No. 858301)

Antigen Details

Structure
CSTB is a 98 amino acid protein with predicted molecular mass of ~11 kD and observed molecular mass of ~14 kD.
Distribution

Tissue Distribution: Predominantly expressed in the tonsil, oral mucosa, and bladder
Cellular Distribution: Cytosol, nucleus, and extracellular

Function
CSTB protects cells from oxidative stress and injury from lysosomal protease leakage.
Interaction
CSTB is an inhibitor of cysteine proteinases such as papain and cathepsin B, cathepsin H, and cathepsin L.
Cell Type
Microglia, Neurons
Biology Area
Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
APP/Aβ Degradation, Enzymes and Regulators, Proteases
Antigen References
  1. Skerget K, et al. 2010. J Biol Chem. 29:285.
  2. Tegelberg S, et al. 2012. J Neuropathol Exp Neurol. 71:40.
  3. Žerovnik E, et al. 2019. Cells. 18:8.
Gene ID
25308 View all products for this Gene ID
UniProt
View information about CSTB on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/21/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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