Purified anti-CRMP2 Antibody

Pricing & Availability
Clone
9F (See other available formats)
Other Names
DPYSL2, Collapsin Response Mediator Protein 2, Dihydropyrimidinase-Related Protein 2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
9F_Purified_CRMP2_Antibody_1_072519
Western blot of purified anti-CRMP2 antibody (clone 9F). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 9F_Purified_CRMP2_Antibody_1_072519
    Western blot of purified anti-CRMP2 antibody (clone 9F). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 9F_Purified_CRMP2_Antibody_2_072519
    IHC staining of purified anti-CRMP2 antibody (clone 9F) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 9F_Purified_CRMP2_Antibody_3_072519
    IHC staining of purified anti-CRMP2 antibody (clone 9F) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 9F_Purified_CRMP2_Antibody_4_072519
    IHC staining of purified anti-CRMP2 antibody (clone 9F) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
869001 25 µg £60
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869002 100 µg £158
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Description

CRMP2 is a ubiquitously expressed, microtubule-associated protein. In the central nervous system, CRMP2 forms homotetramers or heterotetramers with other CRMP proteins during development and regulates axonal growth and guidance, neuronal growth cone collapse, and migration. The C-terminal sequence of CRMP2 is highly homologous to that of Tau protein. Phosphorylation of CRMP2 alters its interaction with microtubules and affects its protein scaffolding function. Similar to Tau, CRMP2 is a biochemically prominent component of Neurofibrillary tangles. These finding suggest that CRMP2 may be an etiologically significant protein and a potential therapeutic target for Alzheimer’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
CRMP2 is a 572 amino acid protein with predicted and observed molecular mass of ~ 63 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol, plasma membrane, and cytoskeleton

Function
CRMP2 forms homotetramers or heterotetramers with other CRMP proteins, and regulates axonal growth and guidance, neuronal growth cone collapse, and migration.
Interaction
CRMP2 forms homotetramers and heterotetramers with CRMP1, DPYSL3, DPYSL4 or DPYSL5. It interacts with HTR4, CLN6, and, MICALL1.
Cell Type
Astrocytes, Neurons, Oligodendrocytes
Biology Area
Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Cytoskeletal Proteins, Microfilaments, Microtubules, Scaffold Proteins
Antigen References
  1. Hensley K, et al. 2016. J Alzheimers dis. 53(1):1
  2. Quach TT, et al. 2015. Mol Psychiatry. 20(9):1037
  3. Ip JP, et la. 2014. Neuroscientist. 20(6):589  
Gene ID
1808 View all products for this Gene ID 12934 View all products for this Gene ID 25416 View all products for this Gene ID
UniProt
View information about CRMP2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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