Purified anti-Cre Recombinase Antibody (Previously Covance catalog# PRB-106P)

Pricing & Availability
Clone
Poly9080 (See other available formats)
Other Names
Cyclization recombinase
Previously
Covance Catalog# PRB-106P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
1 reviews
Product Citations
publications
Poly9080_Pure_Cre-Recombinase_Antibody_1_102218
Total cell lysates (15 µg total protein) from untransfected (-) or Cre-recombinase transfected (+) HEK293E cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:2000 dilution) of Purified anti-Cre recombinase Antibody, clone Poly9080, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Lane M: Molecular Weight marker.
  • Poly9080_Pure_Cre-Recombinase_Antibody_1_102218
    Total cell lysates (15 µg total protein) from untransfected (-) or Cre-recombinase transfected (+) HEK293E cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:2000 dilution) of Purified anti-Cre recombinase Antibody, clone Poly9080, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
908002 25 µl £50
Check Availability


Need larger quantities of this item?
Request Bulk Quote
908001 200 µl £205
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Cre-recombinase is a 38 kD DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. This polyclonal antibody was raised against a full-length Cre Recombinase recombinant protein and was subsequently purified using Protein A. It can be used to determine the success of gene manipulations in transgenic animals.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Bacteriophage Cre Recombinase
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution + 1% BSA and 0.1% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Dudok J, et al. 2016. Neurosci Res. S0168-0102(16)00002-X. PubMed
  2. Benedykcinska A, et al. 2016. Dis Model Mech. 9: 211 - 220. PubMed
  3. Anderson T, et al. 2016. Nature. 10.1038/nature18944. PubMed
  4. Heuer A, et al. 2016. Exp Neurol. 282:78-85. PubMed
  5. Dvorakova M, et al. 2016. Sci Rep. 6:38253. PubMed
  6. Twining R, et al. 2017. Nat Neurosci. 20:459-469. PubMed
Publication Library
RRID
AB_2565079 (BioLegend Cat. No. 908001)

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account