Purified anti-COL4α5 Antibody

Pricing & Availability
Clone
Poly28605 (See other available formats)
Other Names
Collagen Type IV Alpha 5 Chain, Collagen Of Basement Membrane, Alpha-5 Chain, Collagen IV, Alpha-5 Polypeptide, DA149D17.3, DA24A23.1, ATS1, CA54, ASLN
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
Poly28605_PURE_COL4a5_Antibody_1_102919
Western blot of purified anti-COL4α5 antibody (Poly28605). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28605_PURE_COL4a5_Antibody_1_102919
    Western blot of purified anti-COL4α5 antibody (Poly28605). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28605_PURE_COL4a5_Antibody_2_102919
    IHC staining of purified anti-COL4α5 antibody (Poly28605) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28605_PURE_COL4a5_Antibody_3_102919
    IHC staining of purified anti-COL4α5 antibody (Poly28605) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28605_PURE_COL4a5_Antibody_4_102919
    IHC staining of purified anti-COL4α5 antibody (Poly28605) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
860501 25 µg £65
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

COL4α5 is a subunit of type IV collagen, which is the major structural component of glomerular basement membranes (GBM). It forms the meshwork in the extracellular matrix with laminins and proteoglycans. It is also involved in the integrin signaling pathway and associated with diseases including X-linked and kidney disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Collagen Type IV Alpha 5 Chain (COL4α5) expressed in E.coli.
Formulation
0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage, aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
COL4α5 is a 1685 amino acid protein with an apparent molecular mass of ~161 kD.
Distribution

Tissue Distribution: Brain, kidney, and lung
Cellular Distribution: Extracellular and membrane

Function
COL4α5 is involved in formation of glomerular basement membranes and regulation of integrin signaling.
Interaction
Laminin, proteoglycans
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience
Molecular Family
Adhesion Molecules
Antigen References
  1. Cosgrove D, et al. 2017. Matrix Biol. 57-58:45-54.
  2. Barua M, et al. 2018. Am J Kidney Dis. 71(3):441-445.
  3. Nozu K, et al. 2019. Clin Exp Nephrol. 23(2):158-168.
Gene ID
1287 View all products for this Gene ID 12830 View all products for this Gene ID 363457 View all products for this Gene ID
UniProt
View information about COL4alpha5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/29/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account