Purified anti-Cleaved PARP (Asp214) Recombinant Antibody

Pricing & Availability
Clone
QA17A17 (See other available formats)
Other Names
PARP1, Poly (ADP-ribose) polymerase, PPOL, ARTD1, ADPRT, EC 2.4.2, ADP-Ribosyltransferase (NAD+;Poly (ADP-Ribose) Polymerase), Poly (ADP-Ribose) Polymerase Family, Member 1, DNA ADP-Ribosyltransferase PARP1, Poly[ADP-Ribose] Synthase 1
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA17A17_PURE_PARP_Antibody_1_092319
Whole cell extracts (15 μg protein) from HeLa cells untreated (-) or treated (+) with 1 μM staurosporine for 3 hours were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL (1:500 dilution) of Purified anti-Cleaved PARP (Asp214) Recombinant Antibody, clone QA17A17, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: molecular weight marker.
  • QA17A17_PURE_PARP_Antibody_1_092319
    Whole cell extracts (15 μg protein) from HeLa cells untreated (-) or treated (+) with 1 μM staurosporine for 3 hours were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL (1:500 dilution) of Purified anti-Cleaved PARP (Asp214) Recombinant Antibody, clone QA17A17, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: molecular weight marker.
  • QA17A17_PURE_PARP_Antibody_2_092319
    HeLa cells untreated (A) or treated (B) with 1 μM staurosporine for 3 hours were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes and blocked with 5% FBS for 30 minutes. Untreated (A) and treated (B) cells were then intracellularly stained with a 1:500 dilution (1.0 μg/mL) of Purified anti-Cleaved PARP (Asp214) Recombinant Antibody, clone QA17A17, overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 1:200 dilution. Nuclei were counterstained with DAPI and the image was captured with 60x objective.
  • QA17A17_PURE_PARP_Antibody_3_092319
    Human T leukemia cell line Jurkat treated (filled histogram) or non-treated (open histogram) with anti-human CD95 (clone EOS9.1) (Cat. No. 305704) overnight, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with Purified anti-Cleaved PARP (Asp214) Recombinant Antibody (clone QA17A17), followed by anti-mouse IgG1 PE.
  • QA17A17_PURE_PARP_Antibody_4_092319
    Human T leukemia cell line Jurkat treated (Right) or non-treated (Left) with Purified anti-human CD95 (clone EOS9.1) (Cat. No. 305704) overnight, stained with Zombie Violet™ (Cat. No. 423114) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with Purified anti-Cleaved PARP (Asp214) Recombinant Antibody, clone QA17A17, followed by anti-mouse IgG1 PE.
Cat # Size Price Quantity Avail. Save
669901 25 µg £57
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669902 100 µg £144
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Description

PARP (ADP-Ribose) Polymerase (PARP) is a 113 kD nuclear protein important for mediating the normal cellular response to DNA damage and repair in response to environmental stress. PARP catalyzes the transfer of ADP-ribose units from NAD⁺ to various nuclear proteins, including topoisomerases, histones, and PARP itself, by poly(ADP-ribosyl)ation. Following DNA damage, this protein is cleaved by ICE-like caspases, such as caspase-3 and -7. In humans, this PARP cleavage occurs between Asp214 and Gly215, yielding an 89 kD (from the carboxy-terminal catalytic domain) and a 24 kD fragment (from the amino-terminal DNA binding domain). PARP is an important regulator of cellular differentiation, proliferation, and tumor transformation, and PARP cleavage is considered a classical characteristic of cells undergoing apoptosis. Additionally, this enzyme may be the site of mutation in Fanconi anemia, and may contribute to the pathophysiology of type I diabetes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Proprietary synthetic peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg/ml is recommended. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The QA17A17 antibody reacts with the 89 kD (cleaved at Asp214) fragment of human PARP1. It does not react with full-length PARP1.

Fixation and permeabilization using the True-Nuclear Buffer Set and Intracellular Staining Permeabilization Wash Buffer (10X) have also been confirmed to work in addition to the True-Phos™ Perm Buffer during in-house testing and development to detect cleaved PARP (Asp214).

Additional Product Notes

Clone QA17A17 was tested for ICC using PFA-fixed cells permeabilized with either methanol or Triton X-100. Both methods facilitated strong staining.

RRID
AB_2814516 (BioLegend Cat. No. 669901)
AB_2814517 (BioLegend Cat. No. 669902)

Antigen Details

Structure
The large fragment of cleaved PARP is an 801 amino acid product with a predicted molecular weight of 89 kD
Distribution

Nuclear

Function
Molecular “nick sensor”; base excision repair; catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture; DNA metabolism; protein modification may enhance or repress transcription
Interaction
Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modified TATA-BP, YY1, Sp1, NF-B, p53 and others
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Satoh M, Lindahl T. 1992. Nature. 356:356. PubMed
  2. Kaufmann S, et al. 1993. Cancer Res. 53:3976. PubMed
  3. Lazebnik Y, et al. 1994. Nature. 371:347. PubMed
  4. D’Amours D, et al. 1999. Biochem J. 342:249. PubMed
  5. Chaitanya G, et al. 2010. Cell Commun Signal. 8:31. PubMed
Gene ID
142 View all products for this Gene ID
UniProt
View information about Cleaved PARP on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09/23/2019

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