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Purified anti-Chk1 Antibody

Pricing & Availability
Clone
P82G1D5 (See other available formats)
Regulatory Status
RUO
Other Names
Chek1, Checkpoint Kinase 1, EC 2.7.11.1, Checkpoint, S. Pombe. Homolog Of, 1, Chk1-S
Isotype
Mouse IgG1, κ
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Product Citations
publications
P82G1D5_PURE_Chk-1_Anitbody_1_100220.png
Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-Chk1 antibody (clone P82G1D5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • P82G1D5_PURE_Chk-1_Anitbody_1_100220.png
    Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-Chk1 antibody (clone P82G1D5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • P82G1D5_PURE_Chk-1_Anitbody_2_100220.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 6 minutes, and blocked with 5% FBS for 30 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:100 dilution) purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 402201) (panel A) or purified anti-Chk1 antibody (clone P82G1D5) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.5 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
  • P82G1D5_PURE_Chk-1_Anitbody_3_100220.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 402201) or purified anti-Chk1 antibody (clone P82G1D5). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a control antibody raised against a separate epitope of Chk1. Lane M: Molecular weight marker.
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940101 25 µg £81
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940102 100 µg £201
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Description

Checkpoint kinase 1, or Chk1, is a protein serine/threonine kinase that is an essential component of the ATR pathway, which links DNA abnormalities and double strand breaks to downstream signaling events. It is activated through phosphorylation by ATR at serine residues 345 and 317.  Once activated, Chk1 phosphorylates downstream effectors of S, G2, and M phases of the cell cycle to induce transcription regulation, energy consumption alterations, cell cycle arrest, and DNA repair. In cells challenged with excessive DNA damage, Chk1 activates apoptotic pathways. Chk1 inhibition is of therapeutic interest for multiple cancers due to synthetic lethality when pharmacological disruption of the protein is combined with radio- or chemosensitization.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human Chk1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on HeLa cells fixed with 4% PFA and permeabilized with either 100% methanol or Triton X-100. Both methods were compatible with Chk1 staining. 

RRID
AB_2888822 (BioLegend Cat. No. 940101)
AB_2888822 (BioLegend Cat. No. 940102)

Antigen Details

Structure
Chk1 is a 476 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

Ubiquitously expressed/Nuclear and vesicular

Function
Protein kinase/Cell cycle regulator
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Jong Y., et al. 2019. J. Bone Oncol. 10:100268.
2. Al-kaabi M. M., et al. 2015. Brit. Journ. Cancer 112:901-11.
3. Zhang Y., et al. 2014. Int. J. Cancer 134(5):1013-23.
4. Hu G., et al. 2020. J Cancer 11(7):1792-99.

Gene ID
1111 View all products for this Gene ID
UniProt
View information about Chk1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/02/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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