Purified anti-CHIP Antibody

Pricing & Availability
Clone
W17190B (See other available formats)
Other Names
CHIP, STIP1 Homology And U-Box Containing Protein 1, Carboxy Terminus Of Hsp70-Interacting Protein, RING-Type E3 Ubiquitin Transferase CHIP, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, Antigen NY-CO-7, Heat Shock Protein A Binding
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17190B_Purified_CHIP_Antibody_1_050919
Western blot of purified anti-CHIP antibody (W17190B). Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human spleen lysate; Lane 3, 7: 10 µg of mouse kidney lysate; Lane 4, 8: 10 µg of rat spleen lysate. The blot was incubated with 0.5 µg/mL of the primary antibody or Rat IgG2b isotype control overnight at 4°C, followed by incubation with HRP-labeled Goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • W17190B_Purified_CHIP_Antibody_1_050919
    Western blot of purified anti-CHIP antibody (W17190B). Lane 1, 5: Molecular weight marker; Lane 2, 6: 20 µg of human spleen lysate; Lane 3, 7: 10 µg of mouse kidney lysate; Lane 4, 8: 10 µg of rat spleen lysate. The blot was incubated with 0.5 µg/mL of the primary antibody or Rat IgG2b isotype control overnight at 4°C, followed by incubation with HRP-labeled Goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • W17190B_Purified_CHIP_Antibody_2_050919
    IHC staining of purified anti-CHIP antibody (W17190B) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Biotin-labeled Donkey anti-rat IgG. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • W17190B_Purified_CHIP_Antibody_3_050919
    IHC staining of purified anti-CHIP antibody (W17190B) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Biotin-labeled Donkey anti-rat IgG. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • W17190B_Purified_CHIP_Antibody_4_050919
    IHC staining of purified anti-CHIP antibody (W17190B) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Biotin-labeled Donkey anti-rat IgG. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
865701 25 µg £60
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865702 100 µg £158
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Description

CHIP is an E3 ubiquitin-protein ligase known to ubiquitinate  misfolded chaperone-bound substrates and target them for proteasomal degradation. Hsp70, Hsc70, and Hsp90  are three chaperone proteins with which CHIP interacts. CHIP mediates polyubiquitination of DNA polymerase beta at 'Lys-41', 'Lys-61' and 'Lys-81', thereby plays a role in base-excision repair. CHIP can also negatively regulate the suppressive function of regulatory T-cells during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. CHIP also negatively regulates TGF-beta signaling by modulating the basal levels of SMAD3 via ubiquitin-mediated degradation. Mutations in CHIP cause spinocerebellar ataxia type 16.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
CHIP is a 303 amino acid protein with an apparent molecular mass of ~ 35 kD.
Distribution

Tissue Distribution: Brain, heart, skeletal muscle, kidney, liver, and spleen
Cellular Distribution: Cytosol, nucleus, and endoplasmic reticulum

Function
CHIP is involved in protein ubiquitination, protein degradation, and neurodegeneration.
Interaction
Ubiquitin, ATXN3, EPHA2, SMAD3, UBE4B, VCP/p97.
Cell Type
Glutamatergic Neurons, Mature Neurons, Neurons, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neurodegeneration, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family
Enzymes and Regulators, Heat Shock Proteins, Postsynaptic proteins, Phospho-Proteins, Protein Modifiers
Antigen References
  1. Zhan S, et al. 2017. Int Rev Immunol. 36(5):300.
  2. Cao Z, et al. 2016. Oncotarget. 7(20):29864.
Gene ID
10273 View all products for this Gene ID 56424 View all products for this Gene ID 287155 View all products for this Gene ID
UniProt
View information about CHIP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/09/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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