Purified anti-BIRC3 (C-IAP2) Antibody

Pricing & Availability
Clone
W17221A (See other available formats)
Other Names
Baculoviral IAP Repeat Containing 3, Inhibitor Of Apoptosis Protein 1, Apoptosis Inhibitor 2, TNFR2-TRAF-Signaling Complex Protein 1, RNF49, MIHC, API2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17221A_PURE_BIRC3_Antibody_1_070519
Whole cell extracts (15 µg protein) from MCF7 (negative control), HaCaT and A431 (reduced expression controls), Daudi, Ramos, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-BIRC3 (c-IAP2) Antibody, clone W17221A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17221A_PURE_BIRC3_Antibody_1_070519
    Whole cell extracts (15 µg protein) from MCF7 (negative control), HaCaT and A431 (reduced expression controls), Daudi, Ramos, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-BIRC3 (c-IAP2) Antibody, clone W17221A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
667701 25 µg £57
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667702 100 µg £144
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Description

Baculoviral IAP Repeat Containing Protein 3, or BIRC3, is a member of the Inhibitor of apoptosis (IAP) family of proteins initially characterized as a component of the TNFα receptor signaling complex. BIRC3 functions as an E3 ubiquitin ligase, polyubiqiutinating target proteins such as RIP kinases, caspases, TRAF1, and BCL10. Loss of BIRC3 results in a significant impairment of TNFα-mediated NF-κB activation signaling cascades.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant protein corresponding to amino acids 355-604 of human BIRC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Application Notes

This clone has not been tested for IP.

This clone failed to test 4% PFA-fixed Ramos cells permabilized with either methanol or Triton X-100; we do not recommend using the clone for ICC.

This clone produced additional bands between ~65 and 95 kD when tested by WB. These bands were also observed with a control antibody raised with a separate epitope. These bands may represent ubiquitinated BIRC3.

Antigen Details

Structure
BIRC3 is a 604 amino acid protein with a predicted molecular weight of 68 kD.
Distribution

Nucleus, cytoplasm/widely expressed

Function
Inhibits apoptosis
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Innate Immunity, Signal Transduction, Ubiquitin/Protein Degradation
Antigen References
  1. Seitz CS, et al. 2000. J. Clin. Invest. 105:253.
  2. Contze DB, et al. 2005. Mol Cel Biol. 25:3384.
  3. Rahal R, et al. 2014. Nat. Med. 20:87.
Gene ID
330 View all products for this Gene ID
UniProt
View information about BIRC3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/05/2019

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