Purified anti-β Catenin 1 Antibody

Pricing & Availability
Clone
15B8 (See other available formats)
Other Names
β-catenin, CTNNB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
15B8_Purified_beta_Catenin_1_Antibody_1_040519.png
Western blot of purified anti-β Catenin 1 antibody (clone 15B8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 15B8_Purified_beta_Catenin_1_Antibody_1_040519.png
    Western blot of purified anti-β Catenin 1 antibody (clone 15B8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 15B8_Purified_beta_Catenin_1_Antibody_2_040519.png
    IHC staining of purified anti-β Catenin 1 antibody (clone 15B8) on formalin-fixed paraffin-embedded mouse colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 15B8_Purified_beta_Catenin_1_Antibody_3_040519.png
    IHC staining of purified anti-β Catenin 1 antibody (clone 15B8) on formalin-fixed paraffin-embedded rat colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 15B8_Purified_beta_Catenin_1_Antibody_4_110119
    Human colon adenocarcinoma cell line SW480 treated (open historgram) or untreated (filled histogram) with tankyrase inhibitor XAV939 for 18 hours, then treated with True-Nuclear™ Transcription FactorBuffer set (Cat. No. 424401) and stained with purified anti-β Catenin 1 (clone 15B8), followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
862601 25 µg £60
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862602 100 µg £158
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Description

β catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. This protein also binds to adenomatous polyposis coli, which is mutated in the adenomatous polyposis of the colon.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICFC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL is suggested. For intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Guo J, et al. 2018. Cell Res. 28:1141 (IHC-P)
  2. Kisoh K, et al. 2017. Mol Neurobiol. 54:7917 (WB)
  3. Uribe RA, et al. 2015. J Cell Biol. 4:815 (IHC-F)
RRID
AB_2801245 (BioLegend Cat. No. 862601)
AB_2801246 (BioLegend Cat. No. 862602)

Antigen Details

Structure
β Catenin is a 781 amino acid protein with an apparent molecular mass of ~ 86 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, nucleus, cytosol, and extracellular

Function
β Catenin is a dual functional protein involved in cell-cell adhesion and gene transcription.
Interaction
E-cadherin/CDH1, LEF1, TCF4, etc.
Antigen References
  1. Dias C, et al. 2014. Nature. 516(7529): 51
  2. Rosiles A, et al. 2016. CNS Neurol Disord Drug Targets. 15(1):3
  3. Na K, et al. 2017. Anticancer Res. 37(6):3249
  4. Zhu P, et al. 2018. Cancer Res Treat. 50(4):1452
Gene ID
1499 View all products for this Gene ID 12387 View all products for this Gene ID 84353 View all products for this Gene ID
UniProt
View information about β Catenin 1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/09/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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