Purified anti-Beclin-1 Antibody

Pricing & Availability
Clone
Poly28614 (See other available formats)
Other Names
BECN1, Protein GT197, Coiled-coil myosin-like BCL2-interacting protein, VPS30, ATG6 Autophagy Related 6 Homolog, Beclin-1-C 35 kDa, Beclin-1-C 37 kD
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly28614_Purified_Beclin-1_Antibody_1_051319
Western blot of purified anti-Beclin-1 antibody (Poly28614). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28614_Purified_Beclin-1_Antibody_1_051319
    Western blot of purified anti-Beclin-1 antibody (Poly28614). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28614_Purified_Beclin-1_Antibody_2_051319
    IHC staining of purified anti-Beclin-1 antibody (Poly28614) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28614_Purified_Beclin-1_Antibody_3_051319
    IHC staining of purified anti-Beclin-1 antibody (Poly28614) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28614_Purified_Beclin-1_Antibody_4_051319
    IHC staining of purified anti-Beclin-1 antibody (Poly28614) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
861401 25 µg £64
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Description

Beclin-1 plays a central role in autophagy and is involved in autophagosome formation. It acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Beclin-1 is necessary for the initiation of autophagy, and it has been shown to be reduced in the brains of patients with Alzheimer’s disease. Reduced Beclin-1 levels lead to decreased autophagy, the accumulation of Aβ peptides, and neurodegeneration in mouse models of Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Beclin-1 (Met1-Thr273) expressed in E. Coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human Beclin-1 is a 450 amino acid protein with a molecular mass of 52 kD.
Distribution

Tissue distribution: Ubiquitous
Cellular distribution: Cytosol, endoplasmic reticulum, Golgi apparatus, endosome, mitochondrion, and nucleus

Function
Beclin-1 is involved in autophagosome formation in autophagy.
Interaction
Interacts with ATG14 and UVRAG, PI3K complex, AMBRA1, GOPC, GRID2, BCK2, BCL2L1; Also interacts with USP10, USP13, VMP1, DAPK1, RAB39A, ATXN3
Cellular targets: N/A
Cell Type
Astrocytes, Microglia, Neurons
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers, Organelle Markers
Antigen References
  1. Kang R, et al. 2011. Cell Death Differ. 18(4):571-80.
  2. Mcknight NC, et al. 2014. PLoS Genet. 10(10):e1004626
  3. Wirawan E, et al. 2012. Autophagy. 8(1):6-17.
Gene ID
8678 View all products for this Gene ID
UniProt
View information about Beclin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/13/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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