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Purified anti-Ataxin-3 Antibody

Pricing & Availability
Clone
1H9-2 (See other available formats)
Regulatory Status
RUO
Other Names
ATX3, ATXN3, MJD, Machado-Joseph disease protein 1, SCA3, Spinocerebellar ataxia type 3 protein, JOS, josephin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1H9-2_Purified_Ataxin-3_Antibody_1_031919
Whole cell extracts (15 µg protein) from HEK293T and MCF-7 cells were resolved on a 4-20% Tris-glycine gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Ataxin-3 Antibody, clone 1H9-2, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
  • 1H9-2_Purified_Ataxin-3_Antibody_1_031919
    Whole cell extracts (15 µg protein) from HEK293T and MCF-7 cells were resolved on a 4-20% Tris-glycine gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Ataxin-3 Antibody, clone 1H9-2, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
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650401 25 µg £85
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650402 100 µg £182
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Description

Ataxin-3 is an ubiquitously expressed protein which contains a poly-glutamine tract. Genetically abnormal expansion of the poly-glutamine repeats results in accumulation of ubiquitinated ataxin-3 protein aggregates and leads to a neurodegenerative disorder, Machado–Joseph disease (MJD). The protein has ubiquitin interacting motifs and a N-terminal Cysteine residue (C14) responsible for its deubiquitinating activity. Ataxin-3 binds and hydrolyzes polyubiquitinated proteins, and interacts with key proteasome components, such as p97/VCP and p45, indicating that Ataxin-3 may play functional roles in ubiquitin-proteasome system. The protein also act as a transcriptional repressor when interacting with transcriptional activators and coactivators.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length Ataxin-3 recombinant protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhou L, et al. 2013. Neuroscience. 23:243. PubMed
Product Citations
  1. Chapman TP, et al. 2019. Front Immunol. 1.454861111. PubMed
  2. Zhou L, et al. 2013. Neuroscience. 23:243. PubMed
RRID
AB_10900236 (BioLegend Cat. No. 650401)
AB_10900992 (BioLegend Cat. No. 650402)

Antigen Details

Structure
364 aa with predicted molecular weight of 42 kD. The protein contains highly polymorphic polyglutamine repeats and has two isoforms which contain 2 or 3 ubiquitin interacting motifs (UIMs).
Distribution

Nuclear

Function
Has transcriptional repression activity through interaction with regulators of transcription and histones. It regulates ER-associated protein degradation and autophagy when interacts with p97/VCP. It also contains deubiquitination activity.
Interaction
Ataxin-3 interacts with ubiquitin, p97/VCP, histone acetyl-transferases (p300, CBP, and PCAF), and histone.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References

1. Kawaguchi Y, et al. 1994. Nat. Genet. 3:213.
2. Mao Y, et al. 2005. P. Natl. Acad. Sci. USA 102:12700.
3. Li F, et al. 2002. J. Biol. Chem. 277:45004.
4. Zhong X, et al. 2006. Hum. Mol. Genet. 15:2409.
5. Tait D, et al. 1998. Hum. Mol. Genet. 7:991.

Gene ID
110616 View all products for this Gene ID
UniProt
View information about Ataxin-3 on UniProt.org

Related FAQs

There are no FAQs for this product.

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Go To Top Version: 5    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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