Purified anti-Annexin A4

Pricing & Availability
Clone
Poly28642 (See other available formats)
Other Names
Annexin A4, ANX4, Placental Anticoagulant Protein II, 35-Beta Calcimedin, Chromobindin-4, HEL-S-274, Proliferation-Inducing Protein 28, ZAP36
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28642_PURE_Annexin-A4_Antibody_1_101719
Western blot of purified anti-Annexin A4 antibody (Poly28642). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28642_PURE_Annexin-A4_Antibody_1_101719
    Western blot of purified anti-Annexin A4 antibody (Poly28642). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28642_PURE_Annexin-A4_Antibody_2_101719
    IHC staining of purified anti-Annexin A4 antibody (Poly28642) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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864201 25 µg £64
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Description

Annexin A4 is a member of the annexin family, which is a family of calcium-dependent phospholipid binding proteins. Based on its similarity with other members of annexin family, annexin A4 is thought to be involved in membrane-related events including membrane fusion, endocytosis and exocytosis. It may also interact with ATP, inhibit the function of Phospholipase 2A, and regulate synthesis of prostaglandin.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Annexin A4 expressed in E.coli.
Formulation
0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Annexin A4 is a 319 amino acid protein with an apparent molecular mass of ~36 kD.
Distribution

Tissue Distribution: Brain, liver, kidney, lung, and gut
Cellular Distribution: Cytosol, nucleus, and membrane

Function
Annexin A4 is involved in endocytosis and exocytosis, and inhibition of PP2A activity.
Interaction
Phospholipid, ATP
Cell Type
Mesenchymal cells
Biology Area
Cancer Biomarkers, Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Enzymes and Regulators
Antigen References
  1. El Kebir D, et al. 2008. J Leuko Biol. 84(3):600.
  2. Kim A, et al. 2010. Expert Opin Ther Targets. 14(9):963-71.
  3. Matsuzaki S, et al. 2014. Expert Opin Ther Targets. 18(4):403-14.
Gene ID
307 View all products for this Gene ID
UniProt
View information about Annexin A4 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/17/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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