- Clone
- Poly28642 (See other available formats)
- Other Names
- Annexin A4, ANX4, Placental Anticoagulant Protein II, 35-Beta Calcimedin, Chromobindin-4, HEL-S-274, Proliferation-Inducing Protein 28, ZAP36
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Annexin A4 antibody (Poly28642). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Annexin A4 antibody (Poly28642) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 864201 | 25 µg | £64 | ||||
Annexin A4 is a member of the annexin family, which is a family of calcium-dependent phospholipid binding proteins. Based on its similarity with other members of annexin family, annexin A4 is thought to be involved in membrane-related events including membrane fusion, endocytosis and exocytosis. It may also interact with ATP, inhibit the function of Phospholipase 2A, and regulate synthesis of prostaglandin.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant Annexin A4 expressed in E.coli.
- Formulation
- 0.01 M PBS, pH 7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
- Application
-
WB - Quality tested
IHC-P - Validated - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Structure
- Annexin A4 is a 319 amino acid protein with an apparent molecular mass of ~36 kD.
- Distribution
-
Tissue Distribution: Brain, liver, kidney, lung, and gut
Cellular Distribution: Cytosol, nucleus, and membrane - Function
- Annexin A4 is involved in endocytosis and exocytosis, and inhibition of PP2A activity.
- Interaction
- Phospholipid, ATP
- Cell Type
- Mesenchymal cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- El Kebir D, et al. 2008. J Leuko Biol. 84(3):600.
- Kim A, et al. 2010. Expert Opin Ther Targets. 14(9):963-71.
- Matsuzaki S, et al. 2014. Expert Opin Ther Targets. 18(4):403-14.
- Gene ID
- 307 View all products for this Gene ID
- UniProt
- View information about Annexin A4 on UniProt.org
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