Purified anti-α-1-Acid Glycoprotein Antibody

Pricing & Availability
Clone
BL28596 (See other available formats)
Other Names
a1AGP, ORM1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_1_052119
Western blot of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 5 µg of mouse liver lysate; Lane 4: 5 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_1_052119
    Western blot of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 5 µg of mouse liver lysate; Lane 4: 5 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_2_052119
    IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_3_052119
    IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • BL28596_Purified_a-1-Acid-Glycoprotein_Antibody_4_052119
    IHC staining of purified anti-α-1-Acid Glycoprotein antibody (clone BL28596) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
859601 25 µg £60
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Description

α-1-Acid Glycoprotein (α1AGP) is a key acute phase plasma protein synthesized by hepatocytes and functions as a transport protein in the blood stream. The levels of α1AGP become elevated in acute inflammation and therefore it is classified as an acute-phase reactant.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant α-1-Acid Glycoprotein (a1AGP) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
α-1-Acid Glycoprotein is a 201 amino acid protein with a predicted molecular mass of 24 kD and observed molecular mass of 40 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol and nucleus

Function
α-1-Acid Glycoprotein is an acute phase plasma protein which functions as transport protein in the blood stream.
Interaction
Synthetic drugs, cytosolic Ca2+, immune response
Cell Type
Mesenchymal cells, Neurons
Biology Area
Immuno-Oncology, Immunology
Molecular Family
Adaptor Proteins, Innate Immune Signaling
Antigen References
  1. Luo Z, et al. 2015. J Physiol Biochem.  71(2):329.
  2. Xu YF, et al. 2018. Comb Chem High Throughput Screen. 21(10):755.
Gene ID
5004 View all products for this Gene ID 18405 View all products for this Gene ID 24614 View all products for this Gene ID
UniProt
View information about alpha-1-Acid Glycoprotein on UniProt.org

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Go To Top Version: 2    Revision Date: 06/17/2019

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