Purified anti-ALDH2 Antibody

Pricing & Availability
Clone
8D4-D6-D11 (See other available formats)
Other Names
ALDH2, Aldehyde Dehydrogenase 2 Family (Mitochondrial)
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
8D4-D6-D11_PURE_ALDH2_Antibody_1_062419
Western blot of purified anti-ALDH2 antibody (clone 8D4-D6-D11). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 8D4-D6-D11_PURE_ALDH2_Antibody_1_062419
    Western blot of purified anti-ALDH2 antibody (clone 8D4-D6-D11). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 8D4-D6-D11_PURE_ALDH2_Antibody_2_062419
    IHC staining of purified anti-ALDH2 antibody (clone 8D4-D6-D11) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 8D4-D6-D11_PURE_ALDH2_Antibody_3_062419
    IHC staining of purified anti-ALDH2 antibody (clone 8D4-D6-D11) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 8D4-D6-D11_PURE_ALDH2_Antibody_4_062419
    IHC staining of purified anti-ALDH2 antibody (clone 8D4-D6-D11) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
866801 25 µg £60
Check Availability


Need larger quantities of this item?
Request Bulk Quote
866802 100 µg £158
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ALDH2 is a member of the aldehyde dehydrogenase family. In the human brain, ALDH2 is the major aldehyde metabolizing enzyme that is essential for the metabolism and detoxification of endogenous and exogenous aldehyde substrates. In line with this, recent studies have reported that ALDH2 level is dysregulated in the brains of patients with Parkinson’s and Alzheimer's diseases, suggesting toxic aldehydes as a contributing factor to neurodegenerative diseases and a potential therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human ALDH2 fragments expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
ALDH2 is a 517 amino acid protein with predicted and observed molecular mass of ~ 56 kD.
Distribution

Tissue Distribution: Mainly expressed in adrenal gland, lung, kidney, and brain
Cellular Distribution: Mitochondrion and cytosol

Function
Aldehyde metabolizing enzyme that is essential for the metabolism and detoxification of endogenous and exogenous aldehyde substrates.
Interaction
Forms homo-tetramers
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Aldehyde Dehydrogenases, Mitochondrial Markers
Antigen References
  1. Chen CH, et al. 2016. Acta. Neurol. Taiwan. 25(4):111-123.
  2. Chauhan G, Debette S. 2016. Curr. Cardiol. Rep. 18(12):124.
  3. Grünblatt E, Riederer P. 2016. J. Neural Transm. (Vienna). 123(2):83-90.
Gene ID
217 View all products for this Gene ID 11669 View all products for this Gene ID 29539 View all products for this Gene ID
UniProt
View information about ALDH2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/24/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account