Purified anti-ALDH1L1 Antibody

Pricing & Availability
Clone
N103/39 (See other available formats)
Other Names
FDH
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N103-39_Purified_ALDH1L1_Antibody_1_112018
Western blot of purified anti-ALDH1L1 antibody (clone N103/39). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 5 µg of mouse liver lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N103-39_Purified_ALDH1L1_Antibody_1_112018
    Western blot of purified anti-ALDH1L1 antibody (clone N103/39). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 5 µg of mouse liver lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
856801 25 µg £47
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856802 100 µg £94
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Description

Aldehyde dehydrogenase family 1 member L1 (ALDH1L1) was originally discovered as an enzyme that catalyzes the conversion of 10-formyltetrahydrofolate to tetrahydrofolate, with NADPH and CO2 as by products. ALDH1L1 is most exclusively expressed by astroglial cells1 implicating these cells as the major components of folate-dependent formate oxidation in the brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-F - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant) formats include immunocytochemistry3 and immunohistochemistry on frozen tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Zuo H, et al. 2018. J Neurosci. 38(9):2359 (IHC-F)
  2. Kidana K, et al. 2018. EMBO Mol Med. 10:e8184 (WB)
  3. Tian E, et al. 2016. Cell Reports. 16:781 (ICC)
RRID
AB_2783463 (BioLegend Cat. No. 856801)
AB_2783464 (BioLegend Cat. No. 856802)

Antigen Details

Structure
ALDH1L1 is a 902 amino acid protein with an apparent molecular mass of ~ 99 kD.
Distribution

Tissue Distribution: Brain, Liver, Colon, and Kidney.
Cellular Distribution: Extracellular, cytosol, and plamsa membrane.

Function
ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate to tetrahydrofolate.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Neymerer V, et al. 1997. Brain Res. 766(1-2): 195
  2. Brandebura AN, et al. 2018. Dev Neurobiol. doi: 10.1002/dneu.22633.
  3. Garwood CJ, et al. 2017. Neuropathol Appl Neurobiol. 43(4):281
Gene ID
10840 View all products for this Gene ID 107747 View all products for this Gene ID
UniProt
View information about ALDH1L1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/21/2018

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