Presynaptic Vesicle Antibody Sampler Kit

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1-N28slash9_PURE_VGlut1_Antibody_WB_071018.png
Western blot of purified anti-VGlut1 antibody (clone N28/9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1-N28slash9_PURE_VGlut1_Antibody_WB_071018.png
    Western blot of purified anti-VGlut1 antibody (clone N28/9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2-A17080A_PURE_Synapsin_Antibody_WB_072018.png
    Western blot of purified anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 0.005 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3-A17080A_PURE_Synapsin_Antibody_IHC-P_2_072018.png
    IHC staining of purified anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 4-N356.9_Purfied_SVOP_2_IHC_020615
    IHC staining of purified anti-SVOP antibody (clone N356/9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective
  • 5-SP8_PURE_Syntaxin_Antibody_IHC_2_RB_051818.png
    IHC staining of purified anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 6-SMI-81_Pure_SNAP25_Antibody_1_081718.png
    IHC staining of purified anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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899909 1 kit £232
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Description

The Synaptic Vesicle Antibody Sampler Kit offers flexibility for sampling and detection of proteins involved in glutamate transport, synaptic vesicle membrane fusion, and neurotransmitter release. These antibodies are highly suitable for WB and IHC applications.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype
Anti-VGlut1 N28/9 25 μl Human, Mouse, Rat Mouse IgG1, κ
Anti-Synapsin I/II/III  A17080A 25 μg Human, Rat Mouse IgG2b, κ
 Anti-SVOP  N356/9 25 μl Mouse, Rat, Human Mouse IgG2b
 Anti-Syntaxin SP8 25 μl Human, Mouse, Rat Mouse IgG1
 Anti-SNAP-25 SMI 81 25 μg Human, Mouse, Rat Mouse IgG1, κ

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Antibody Type
Monoclonal
Formulation
Please refer to individual product datasheets of the purified formats for details.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB, IHC-P - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-VGlut1: 1.0 - 10 µg/ml
Anti-Synapsin I/II/III: 0.001 - 0.005 µg/ml

For immunohistochemistry on formalin-fixed paraffin-embedded tissue, the suggested uses of these reagents are as follows:

Anti-SVOP: 10 µg/ml
Anti-Synapsin I/II/III: 0.1 - 0.5 µg/ml
Anti-Syntaxin: 0.5 - 5.0 µg/ml
Anti-SNAP-25: 0.1 - 1.0 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

Related FAQs

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Go To Top Version: 1    Revision Date: 10/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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