PerCP/Cyanine5.5 anti-mouse TCR Vγ2 Antibody

Pricing & Availability
Clone
UC3-10A6 (See other available formats)
Other Names
Vγ2 T-cell receptor, T cell receptor gamma 2
Isotype
Armenian hamster IgG
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Product Citations
publications
UC3-10A6_PERCP_CYANINE5-5_TCRVgamma2_Antibody_090619
C57BL/6 splenocytes were stained with CD3 FITC and TCR Vγ2 (clone UC3-10A6) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • UC3-10A6_PERCP_CYANINE5-5_TCRVgamma2_Antibody_090619
    C57BL/6 splenocytes were stained with CD3 FITC and TCR Vγ2 (clone UC3-10A6) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
137711 25 µg £77
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137712 100 µg £205
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Description

T cell receptor (TCR) Vγ2 bearing T lymphocytes make up a significant proportion of γδ TCR cells in late fetal and adult peripheral lymphoid tissues. TCR γδ T cells may play a role in immunological surveillance for stress-induced self-antigens. The frequency of Vγ2 expression in different strains varied from 12% to 54% in the TCR γδ repertoire. Variations in the levels of Vγ2+ cells are not associated with MHC haplotype. High Vγ2 expression is influenced by the TCR-δ locus.  Expanding Vγ2+ TCRγδ cells in B6 mice overwhelmingly use a Vδ7+ δ chain except in the DBA/2 strain.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
G8 mouse T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Dent AL, et al. 1990. Nature 343:714.
  2. Kelly KA, et al. 1993. Int. Immunol. 5:331.
  3. Sperling AI, et al. 1992. J. Immunol. 149:3200.
  4. Sperling AI, et al. 1997. J. Immunol. 159:86.
RRID
AB_2814039 (BioLegend Cat. No. 137711)
AB_2814040 (BioLegend Cat. No. 137712)

Antigen Details

Structure
A member of Ig superfamily, associated with TCR δ chain and CD3 complex.
Distribution

T cell subset in fetal and adult peripheral lymphoid tissues and thymus.

Function
Might play a role in a previously unrecognized form of immunological surveillance for stress-induced self-antigens.
Ligand/Receptor
Antigens
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679.
2. O’Brien RL, et al. 2000. J. Immunol. 165:6472.

Gene ID
110067 View all products for this Gene ID
UniProt
View information about TCR V gamma2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 09/06/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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