PerCP/Cyanine5.5 anti-mouse TCR Vβ11 Antibody

Pricing & Availability
Clone
KT11 (See other available formats)
Other Names
T cell receptor Vβ 11 [vb11]
Isotype
Rat IgG2b, κ
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Product Citations
publications
KT11_PerCPCyanine55_TCR_Vbeta11_Antibody_010419.png
C57BL/6 splenocytes were stained with CD3 (clone 145-2C11) FITC and TCR Vβ11 (clone KT11) PerCP/Cyanine5.5 (left) or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • KT11_PerCPCyanine55_TCR_Vbeta11_Antibody_010419.png
    C57BL/6 splenocytes were stained with CD3 (clone 145-2C11) FITC and TCR Vβ11 (clone KT11) PerCP/Cyanine5.5 (left) or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
125911 25 µg £77
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125912 100 µg £202
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Description

The Vβ11 gene locus is deleted in mice of TCR a haplotype (C57BR, C57L, SJL, SWR) and TCR c haplotype (RIII).Mice of TCR b haplotype (C57BL, BALB/c, AKR, etc) have Vβ11-bearing T cells .However, mice expressing MHC class II I-E and some superantigens, such as Mtv-8, Mtv-9 and Mtv-11 will show a partial deletion of Tb11-bearing T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Quigley MF, et al. 2010. Proc Natl Acad Sci USA. 9:19414. PubMed

Antigen Details

Distribution

Positive on a subset of alpha/beta T cells

Ligand Receptor
MHC/peptide
Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta11 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 01/04/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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