PerCP/Cyanine5.5 anti-mouse CD98 (4F2) Antibody

Pricing & Availability
Clone
RL388 (See other available formats)
Other Names
4F2, Ly10,
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RL388_PerCPCyanine55_CD98_Antibody_121018
C57BL/6 splenocytes were stained with CD98 (clone RL388) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • RL388_PerCPCyanine55_CD98_Antibody_121018
    C57BL/6 splenocytes were stained with CD98 (clone RL388) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
128217 25 µg £97
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128218 100 µg £198
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Description

CD98 is a cell surface glycoprotein of 125 kd consisting of an 85 kd heavy chain and a 40 kd light chain.It is expressed on T, B, NK cells, granulocytes and many cell lines and tumor cells.CD98 has been shown to be involved in sodium-dependent calcium flux in muscles cells and lymphocyte activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Subline of mouse T lymphoma EL4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Luscher B, et al. 1985. J. Immunol. 135 :3951
  2. MacDonald HR, et al. 1985. J. Immunol. 135 :3944
RRID
AB_2783081 (BioLegend Cat. No. 128217)
AB_2783082 (BioLegend Cat. No. 128218)

Antigen Details

Structure
Cell surface glycoprotein of 125 kd consisting of an 85 Kd heavy chain and a 40 kd light chain.
Distribution

Expressed on T cells, B cells , NK cells, granulocytes, etc.

Function
Regulatory role in amino acid transport system. Modulate sodium-dependent calcium flux and cell activation
Cell Type
B cells, Granulocytes, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Luscher B, et al. 1985. J. Immunol. 135 :3951
2. MacDonald HR, et al. 1985. J. Immunol. 135 :3944

Gene ID
17254 View all products for this Gene ID
UniProt
View information about CD98 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 12/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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