PerCP/Cyanine5.5 anti-mouse CD94 Antibody

Pricing & Availability
Clone
18d3 (See other available formats)
Other Names
Kp43
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
18d3_PerCPCyanine55_CD94_Antibody_051019
C57BL/6 mouse splenocytes were stained for CD49b FITC and CD94 (clone 18d3) PerCP/Cyanine5.5 (left) or rat IgG2 a, κ isotype control (right).
  • 18d3_PerCPCyanine55_CD94_Antibody_051019
    C57BL/6 mouse splenocytes were stained for CD49b FITC and CD94 (clone 18d3) PerCP/Cyanine5.5 (left) or rat IgG2 a, κ isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
105513 25 µg £111
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105514 100 µg £235
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Description

CD94 is a 43/39 kD C-type lectin, also known as Kp43. It is present on all NK cells, NKT cells, and a subset of CD8-positive T lymphocytes in most mouse strains. CD94 is a type-II transmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail. CD94 is expressed as a disulphide-linked heterodimer with a NKG2 subunit believed to mediate signal transduction. When associated with NKG2A, the complex triggers inhibition; when associated with NKG2C, the complex triggers stimulation. The receptor complex of CD94 and NKG2 receptors bind to the ligand, Qa-1, and are thought to play a role in maintaining self-tolerance in developing NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells expressing the C57BL/6 alleles of CD94 and NKG2A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
  2. Tang X, et al. 2006. J. Immunol. 177:7645. PubMed
  3. This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
C-type lectin; 43/39 kD
Distribution

NK cells, NK-T cells, CD8+ T subset

Function
Inhibits NK cell function
Ligand/Receptor
CD94/NKG2
Cell Type
NK cells, NKT cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165.
  3. Moretta A, et al. 1997. Immunol. Rev. 155:105.
  4. Phillips JH, et al. 1996. Immunity 5:163.
Gene ID
16643 View all products for this Gene ID
UniProt
View information about CD94 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 05/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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