PerCP/Cyanine5.5 anti-mouse α-GalCer:CD1d complex Antibody

Pricing & Availability
Clone
L363 (See other available formats)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
L363_PerCPCy55_alpha-GalCer-Cd1d_Antibody_101218
Murine CD1d transfected RMA-s cells were treated with (filled histogram) or without (open histogram) α-GalCer overnight, then stained with α-GalCer:CD1d complex (clone L363) PerCP/Cyanine5.5.
  • L363_PerCPCy55_alpha-GalCer-Cd1d_Antibody_101218
    Murine CD1d transfected RMA-s cells were treated with (filled histogram) or without (open histogram) α-GalCer overnight, then stained with α-GalCer:CD1d complex (clone L363) PerCP/Cyanine5.5.
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
140513 25 µg £101
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140514 100 µg £225
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Description

The L363 antibody reacts with α-GalCer:mouse CD1d complex. Glycolipid α-galactosylceramide (α-GalCer) is a potent activator of human and murine iNKT cells and is widely used as a model antigen for the studies of iNKT-mediated immunity. The glycolipid binds to CD1d and forms a complex which is subsequently recognized by iNKT cells, a subset of T cells expressing invariant TCR α chain Vα14, analogous to the peptide-MHC complex recognition by TCR α/ß T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
α-GalCer:mCD1d1 complexes coupled to Mycobacterium tuberculosis PPD
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Surface and intracellular immunofluorescence2 and block α-GalCer:mCD1d1 complex induced iNKT activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Yu KO, et al. 2007. J. Immunol. Methods 323:11. (Block)
  2. Im JS, et al. 2009. Immunity 30:888. (IF)
RRID
AB_2783101 (BioLegend Cat. No. 140513)
AB_2783102 (BioLegend Cat. No. 140514)

Antigen Details

Distribution

NKT cells

Cell Type
B cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens, TCRs
Antigen References

1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.
5. Sullivan BA and Kronenberg M. 2005. J. Clin. Invest. 115:2328.

Gene ID
12479 View all products for this Gene ID
UniProt
View information about alpha-GalCer-CD1d complex on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10/15/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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