PerCP/Cyanine5.5 anti-human IL-21 Antibody

Pricing & Availability
Clone
3A3-N2 (See other available formats)
Other Names
Interleukin 21
Isotype
Mouse IgG1, κ
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Product Citations
publications
3A3-N2_PerCPCyanine55_IL-21_Antibody_102119
PMA/ionomycin-stimulated (4 hours) human peripheral blood lymphocytes intracellular stained with IL21 (clone 3A3-N2) PerCP/Cyanine5.5 (left) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right) and CD3 APC.
  • 3A3-N2_PerCPCyanine55_IL-21_Antibody_102119
    PMA/ionomycin-stimulated (4 hours) human peripheral blood lymphocytes intracellular stained with IL21 (clone 3A3-N2) PerCP/Cyanine5.5 (left) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right) and CD3 APC.
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
513011 25 tests £106
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513012 100 tests £250
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Description

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset, and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells.  It can also be proapoptotic for B cells and NK cells. Studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation, suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human IL-21
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)

Antigen Details

Distribution

Th17

Function
Immunomodulation
Cell Sources
NK-T cells and T cells
Cell Targets
Cytotoxic T cells (CTL) and Natural Killer (NK) cells that can destroy malignant or infected cells
Receptors
IL-21R
Cell Type
Th17
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nurieva R. 2007. Nature 448:416.
2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao H, et al. 2001. J. Immunol. 167:1.
5. Parrish-Novak J, et al. 2000. Nature 408:57.

Gene ID
59067 View all products for this Gene ID
UniProt
View information about IL-21 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10/14/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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