Human peripheral blood platelets were stained with CD49b (clone P1E6-C5) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Human peripheral blood platelets were stained with CD49b (clone P1E6-C5) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
CD49b is a 170 kD transmembrane protein, also known as α2 integrin, VLA-2 α chain, Integrin α2 and GPIa. It associates with CD29 (β1 integrin) to form VLA-2, a collagen and laminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser
Blue Laser (488 nm)
Application Notes
Additional reported applications (for the relevant formats of this clone) include: in vitro blocking activity1,2, immunoprecipitation3, and ELISA4.
Application References
(PubMed link indicates BioLegend citation)
Hirsch MS, et al. 1997. Dev. Dyn. 210:249. (Block)
Sawhney RS, et al. 2006. J. Biol. Chem. 281:8497. (Block)
Lee SA, et al. 2009. Carcinogensis. 30:1872. (IP)
Zßrate S, et al. 2004. J. Virol. 78:10839. (ELISA)
Integrin alpha chain family, type I membrane protein, alpha chain of heterodimeric integrin receptor, 170 kD; associates with CD29 to form collagen, laminin receptor
Distribution
Broadly expressed on many cell types including B cells, monocytes, platelets, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts
Function
With CD29; binds collagen and laminin
Ligand/Receptor
Forms heterodimer with integrin β1 (CD29) which is a receptor for laminin and collagen
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
1. Kaplan C, et al. 1991. Br. J. Haematol. 78:425.
2. Kiefel V, et al. 1991. Vox Sang. 60:244.
3. Nieuwenhuis HK, et al. 1985. Nature 318:470.
4. Takada Y and Helmer ME. 1989. J. Cell Biol. 109:397.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
