PerCP/Cyanine5.5 anti-human CD355 (CRTAM) Antibody

Pricing & Availability
Clone
Cr24.1 (See other available formats)
Other Names
Class I-MHC-Restricted T-cell Associated Molecule
Isotype
Mouse IgG2a, κ
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Product Citations
publications
Cr24dot1_PerCPCyanine55_CD355_Antibody_110618
PMA/Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were stained with CD8a (clone RPA-T8) APC and CD355 (clone Cr24.1) PerCP/Cyanine5.5 (left) or Mouse IgG2a, κ PerCP/Cyanine5.5 Isotype Control (right).
  • Cr24dot1_PerCPCyanine55_CD355_Antibody_110618
    PMA/Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were stained with CD8a (clone RPA-T8) APC and CD355 (clone Cr24.1) PerCP/Cyanine5.5 (left) or Mouse IgG2a, κ PerCP/Cyanine5.5 Isotype Control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
339111 25 tests £101
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339112 100 tests £235
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Description

CRTAM, (class-I MHC-restricted T-cell associated molecule), is homodimer protein belonging to Ig superfamily. It is expressed transiently on activated NK cells,NKT cells and a subset of CD8+ T cells. Nectin-like molecule-2 (Necl2, TSCL1) is the ligand of CRTAM. CRTAM plays an important role in cell adhesion and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CRTAM-P815 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kent S, et al. 2005. Blood. 106:779
RRID
AB_2783221 (BioLegend Cat. No. 339111)
AB_2783222 (BioLegend Cat. No. 339112)

Antigen Details

Structure
Dimer, Ig superfamily
Distribution

Activated NK cells and subset of CD8+ cells

Function
Adhesion and migration
Ligand Receptor
Nectin-like molecule 2 (Necl2)
Cell Type
NK cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, MHC Antigens
Antigen References

1. Arase N, et al. 2005. Intl. Immunol. 17:1227
2. Kennedy J, et al. 2000. J. Leukoc. Biol. 67:725

Gene ID
56253 View all products for this Gene ID
UniProt
View information about CD355 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/06/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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