PerCP/Cyanine5.5 anti-human CD328 (Siglec-7) Antibody

Pricing & Availability
Clone
6-434 (See other available formats)
Workshop
VIII 80652
Other Names
Siglec7 (Sialic-binding Ig like lectin 7), p75, AIRM1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6-434_PERCP_CYANINE55_CD328_Antibody_081619
Human peripheral blood lymphocytes were stained with CD56 Brilliant Violet™ 421 and CD328 (Siglec-7) (clone 6-434) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • 6-434_PERCP_CYANINE55_CD328_Antibody_081619
    Human peripheral blood lymphocytes were stained with CD56 Brilliant Violet™ 421 and CD328 (Siglec-7) (clone 6-434) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
339215 25 tests £101
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339216 100 tests £232
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Description

Siglec-7, also known as p75/AIRM1, is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs). It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2814250 (BioLegend Cat. No. 339215)
AB_2814251 (BioLegend Cat. No. 339216)

Antigen Details

Structure
Type I transmembrane protein, Siglec family member, 75 kD
Distribution

NK cells, monocytes

Function
Inhibitory receptor of NK cells
Ligand/Receptor
Sialylated glycans (with α2,8 disialyl and α2,6 sialyl residues)
Cell Type
Monocytes, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules, Siglec Molecules
Antigen References
  1. Avril T, et al. 2006. Infection and Immunity 74:4133
  2. Avril T, et al. 2004. J. Immunol. 173:6841
  3. Yamaji T, et al. 2005. Glycobiology 15:667
Gene ID
27036 View all products for this Gene ID
UniProt
View information about CD328 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 08/16/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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