PerCP/Cyanine5.5 anti-human CD307d (FcRL4) Antibody

Pricing & Availability
Clone
413D12 (See other available formats)
Other Names
FcRL4 (Fc receptor-like 4), IRTA1, IGFP2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
413D12_PerCPCyanine55_CD307d_Antibody_082719
Human FcRL4 transfected P815 cells were stained with CD307d (FcRL4) (clone 413D12) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 413D12_PerCPCyanine55_CD307d_Antibody_082719
    Human FcRL4 transfected P815 cells were stained with CD307d (FcRL4) (clone 413D12) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
340211 25 tests £94
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340212 100 tests £242
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Description

FcRL4 (Fc receptor-like 4) is a 57 kD single pass type I transmembrane protein that exhibits 4 Ig like C2 domains. Also known as ITRA1, IGFP2, and FcRH4, FcRL4 is predominately found on a subset of memory B cells. Activation of FcRL4 contributes to B cell receptor inhibition through activation of the tyrosine kinases SHP-1 and 2. FcRL4's nascent loci is 1q23.1; however, breakpoint mutations are associated with multiple myeloma and non-Hodgkins lymphoma. Increased FcRL4 expression on memory B cells has also been observed in HIV viremic patients. FcRL4 predominately binds aggregated IgA, binds weakly with IgG3 and IgG4, and does not bind IgG1 and IgG2. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
P815 cells transfected with FcRL4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (with relevant formats) include: immunoprecipitation and blocking. mAb 413D12 is able to block the binding of FcRL4 to aggregated IgA, IgG3, and IgG4.

Application References

(PubMed link indicates BioLegend citation)
  1. Sohn HW, et al. 2011. Blood. 118:6332. PubMed
  2. Wilson TJ, et al. 2012. J. Immunol. 188:4741. (Block)
RRID
AB_2814253 (BioLegend Cat. No. 340211)
AB_2814254 (BioLegend Cat. No. 340212)

Antigen Details

Structure
57 kD, Fc receptor like, four Ig like domains
Distribution

Subset of memory B cells, some multiple myeloma and non-Hodgkins lymphoma

Interaction
Tyrosine phophorylated FcRL4 interacts with SHP-1, SHP-2 to inhibit the B cell receptor
Bioactivity
B-cell receptor inhibition
Cell Type
B cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Moir S, et al. 2008. J. Exp. Med. 205:1797.
2. Ehrhardt GRA, et al. 2003. P. Natl. Acad. Sci. USA 100:13489.
3. Ehrhardt GRA, et al. 2005. J. Exp. Med. 202:783.
4. Polson AG, et al. 2006. J. Int. Immunol. 18:1363.

Gene ID
83417 View all products for this Gene ID
UniProt
View information about FcRL4 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 08/27/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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