PerCP/Cyanine5.5 anti-human CD298 Antibody

Pricing & Availability
Clone
LNH-94 (See other available formats)
Other Names
Na+/K+ ATPase β3, ATP1B3
Isotype
Mouse IgG1, κ
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Product Citations
publications
LNH-94_PerCPCyanine55_CD298_Antibody_051319
Human peripheral blood lymphocytes were stained with CD298 (clone LNH-94) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • LNH-94_PerCPCyanine55_CD298_Antibody_051319
    Human peripheral blood lymphocytes were stained with CD298 (clone LNH-94) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
341709 25 tests £114
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341710 100 tests £242
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Description

CD298 or the β3 Na+/K+ ATPase, is a 42 kD type II transmembrane protein, also known as ATP1B3. An integral plasma membrane protein, Na+/K+ ATPase is composed of one α and one β subunits. Four isoforms of the α and three isoforms of the β subunits have been reported. Na+/K+ ATPase couples ATP hydrolysis to the development of an ionic gradient by pumping Na+ and K+ ions in opposite directions across the cell plasma membrane. It has broad tissue distribution, including all leukocytes and many other tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)

Antigen Details

Structure
CD298 is the β3 subunit of Na+/K+ ATPase, type II transmembrane protein
Distribution

Broad expression including all leukocytes and other tissues

Function
sodium-potassium pump, moves these Na+ and K+ ions in opposite directions across cell plasma membrane
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Chiampanichayakul S, et al. 2006. Tissue Antigens. 68:509
3. Malik N, et al. 1996. J. Biol. Chem. 271:22754

Gene ID
483 View all products for this Gene ID
UniProt
View information about CD298 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 05/13/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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