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PerCP/Cyanine5.5 anti-STAT6 Phospho (Tyr641) Antibody

Pricing & Availability
Clone
A15137E (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 6, IL-4 Stat
Isotype
Mouse IgG1, κ
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Product Citations
publications
A15137E_PerCPCy5.5_STAT6_Phospho_Antibody_103116
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with STAT6 Phospho (Tyr 641) (clone A15137E) PerCP/Cyanine5.5.
  • A15137E_PerCPCy5.5_STAT6_Phospho_Antibody_103116
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with STAT6 Phospho (Tyr 641) (clone A15137E) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
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686009 25 tests £109
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686010 100 tests £278
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Description

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, translocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT6 peptide phosphorylated at Tyr 641
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Human STAT6 has three isoforms; the molecular weights are 94, 82 and 74kD. The immunogen (phosphorylated peptide) is shared by these three isoforms. This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms. The predominant band detected is at 94 kD.

Clone A15137E does not react with mouse.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

RRID
AB_2632682 (BioLegend Cat. No. 686009)
AB_2632683 (BioLegend Cat. No. 686010)

Antigen Details

Structure
847 amino acids with a predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.
Distribution

Cytoplasm; translocates to the nucleus when phosphorylated.

Function
STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling. While STAT6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. Following phosphorylation by Janus kinases, STAT6 translocates to the nucleus where it regulates gene transcription.
Interaction
STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References

1. Goenka S, et al. 2011. Immunol. Res. 50:87.
2. Wurster AL, et al. 2000. Oncogene 19:2577.
3. Akira S. 1999. Stem Cells 17:138.
4. Zamorano J, et al. 2005. J. Immunol. 174:2843.
5. David M, et al. 2001. Oncogene 20:6660.
6. Takeda K, et al. 1996. Nature 380:627.

Gene ID
6778 View all products for this Gene ID
UniProt
View information about STAT6 Phospho Tyr641 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10/31/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-STAT6 Phospho (Tyr641)

  • PE anti-STAT6 Phospho (Tyr641)

  • PerCP/Cyanine5.5 anti-STAT6 Phospho (Tyr641)

  • Alexa Fluor® 488 anti-STAT6 Phospho (Tyr641)

  • PE/Cyanine7 anti-STAT6 Phospho (Tyr641)

  • Alexa Fluor® 647 anti-STAT6 Phospho (Tyr641)

  • Direct-Blot™ HRP anti-STAT6 Phospho (Tyr641)

  • APC anti-STAT6 Phospho (Tyr641)

  • Brilliant Violet 421™ anti-STAT6 Phospho (Tyr641)
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