- 53-5.8 (See other available formats)
- Regulatory Status
- Other Names
- Lyt-3.2, Ly-3.2
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse thymus or spleen
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.
(PubMed link indicates BioLegend citation)
- Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
- Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
- Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
- Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
- Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
- Product Citations
AB_2800650 (BioLegend Cat. No. 140417)
AB_2800651 (BioLegend Cat. No. 140418)
- Ig superfamily, CD8β chain, 32 kD
Majority of thymocytes, cytotoxic T cell subsets
- Co-receptor of TCR
- MHC class I
- Cell Type
- T cells, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules, TCRs
- Antigen References
1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439.
- Gene ID
- 12526 View all products for this Gene ID
- View information about CD8b.2 on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-mouse CD8b.2||53-5.8||FC,ICC,IHC-F,IP|
|FITC anti-mouse CD8b.2||53-5.8||FC|
|Biotin anti-mouse CD8b.2||53-5.8||FC|
|PE anti-mouse CD8b.2||53-5.8||FC|
|APC anti-mouse CD8b.2||53-5.8||FC|
|Pacific Blue™ anti-mouse CD8b.2||53-5.8||FC|
|PE/Cyanine7 anti-mouse CD8b.2||53-5.8||FC|
|PerCP/Cyanine5.5 anti-mouse CD8b.2||53-5.8||FC|
|APC/Fire™ 750 anti-mouse CD8b.2||53-5.8||FC|
|APC/Cyanine7 anti-mouse CD8b.2||53-5.8||FC|
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