- MIH35 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2a, κ
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- Product Citations
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B7-H3 is a type I transmembrane protein belonging to the B7 family of co-stimulatory proteins. B7-H3 is mostly expressed on professional APCs including B cells, macrophages, and dendritic cells at low levels. It is detected on various human and murine tumor cells, nasal and airway epithelial cells. Its expression on dendritic cells appears to be up-regulated by LPS. Initial studies have shown that B7-H3 provides a stimulatory signal to T cells. However, recent studies suggest a negative regulatory role for B7-H3 in T cell responses. Mouse B7-H3 protein inhibited T cell activation and effector cytokine production. Thus, the immunological function of B7-H3 remains unclear. B7-H3 is involved in the suppression of Th1-mediated immune responses and plays an important role in the development of pathogenic Th2 cells in a murine asthma model. Monoclonal antibody against B7-H3 enhances T cell proliferation in vitro and leads to exacerbated EAE in vivo. It has been reported that the Triggering Receptor Expressed on Myeloid cells (TREM)-like Transcript 2 (TLT-2, TREML2) is a receptor for B7-H3 in mice, although it remains controversial. Further studies are needed to identify the receptor of B7-H3.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse B7-H3 transfected L cell and P815
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
(PubMed link indicates BioLegend citation)
- Hashiguchi M, et al. 2008. Proc Natl Acad Sci USA. 105(30):10495.
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
AB_2800638 (BioLegend Cat. No. 135615)
AB_2800639 (BioLegend Cat. No. 135616)
- A 49.7 kD member of the B7 family of the Ig superfamily proteins.
Mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells at low level.
- Provides a stimulatory signal to T cells, plays a negative regulatory role in T cell responses as well.
- The receptor has not been completely identified, but it seems to be expressed on activated T cells.
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
1. Nagashima O, et al. 2008. J. Immunol. 181:4062
2. Prasad DVR, et al. 2004. J. Immunol. 173:2500
3. Sun M, et al. 2002. J. Immunol. 168:6294
4. Xu J, et al. 2006. Cellular and Molecular Immunology. 3(3):235
5. Ford JW, et al. 2009. Curr Opin Immunol. 21(1):38
6. Leitner J, et al. 2009. Eur. J. Immunol. 2009. 39(7):1754
- Gene ID
- 102657 View all products for this Gene ID
- View information about CD276 on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-mouse CD276 (B7-H3)||MIH35||FC|
|PE anti-mouse CD276 (B7-H3)||MIH35||FC|
|APC anti-mouse CD276 (B7-H3)||MIH35||FC|
|Biotin anti-mouse CD276 (B7-H3)||MIH35||FC|
|PE/Dazzle™ 594 anti-mouse CD276 (B7-H3)||MIH35||FC|
|PE/Cyanine7 anti-mouse CD276 (B7-H3)||MIH35||FC|
|PerCP/Cyanine5.5 anti-mouse CD276 (B7-H3)||MIH35||FC|
|Ultra-LEAF™ Purified anti-mouse CD276 (B7-H3)||MIH35||FC, FA|
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