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PerCP/Cyanine5.5 anti-human Ig light chain λ Antibody

Pricing & Availability
Clone
MHL-38 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin light chain lambda
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MHL-38_PerCPCyanine55_light_chain_gamma_Antibody_083018
Human peripheral blood lymphocytes were stained with CD19 PE and Ig light chain λ (Clone MHL-38) PerCP/Cyanine5.5 (left), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • MHL-38_PerCPCyanine55_light_chain_gamma_Antibody_083018
    Human peripheral blood lymphocytes were stained with CD19 PE and Ig light chain λ (Clone MHL-38) PerCP/Cyanine5.5 (left), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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316617 25 tests £97
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316618 100 tests £238
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Description

The MHL-38 antibody reacts with both soluble and membrane human immunoglobulin light chain lambda (λ). It does not react with human immunoglobulin light chain kappa (κ) or heavy chains. The MHL-38 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone MHL-38 is not compatible with Human TruStain FcX (Fc Receptor Blocking Solution) (Cat. No. 422301 & 422302).

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Product Citations
  1. Poran A, et al. 2020. Cell Reports Medicine. 1(8):100141. PubMed
RRID
AB_2561511 (BioLegend Cat. No. 316617)
AB_2561512 (BioLegend Cat. No. 316618)

Antigen Details

Structure
Ig family
Distribution

B cells

Cell Type
B cells
Biology Area
Immunology
Gene ID
3535 View all products for this Gene ID
UniProt
View information about Ig light chain lambda on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 08/30/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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