PerCP/Cyanine5.5 anti-human CD235a (Glycophorin A) Antibody

Pricing & Availability
Clone
HI264 (See other available formats)
Workshop
VII 70312
Other Names
PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, MNS blood group
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
HI264_PERCPCy5.5_CD235a_Antibody_FC_102813
Human red blood cells were stained with anti-human CD235a (clone HI264) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • HI264_PERCPCy5.5_CD235a_Antibody_FC_102813
    Human red blood cells were stained with anti-human CD235a (clone HI264) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2a, ? PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
349109 25 tests £103
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349110 100 tests £239
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Description

CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes.  It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
Product Citations
  1. Nguyen M et al. 2018. Cell reports. 25(13):3884-3893 . PubMed
  2. Kotini AG et al. 2017. Cell stem cell. 20(3):315-328 . PubMed
  3. Pelon F, et al. 2020. Nat Commun. 0.738888889. PubMed
  4. Kieffer Y, et al. 2020. Cancer Discov. 1.340277778. PubMed
  5. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
RRID
AB_2562705 (BioLegend Cat. No. 349109)
AB_2562706 (BioLegend Cat. No. 349110)

Antigen Details

Structure
Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer
Distribution

Erythrocytes and erythroid precursors

Function
Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
Ligand/Receptor
Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
Cell Type
Erythrocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Reid ME. 2009. Immunohematology 25:95.
2. Palacajornsuk P. 2006. Immunohematology 22:171.
3. Pasvol G. 2003. Trends Parasitol. 19:430.
4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80.

Gene ID
2993 View all products for this Gene ID
UniProt
View information about CD235a on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 05/24/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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