PerCP/Cyanine5.5 anti-human CD163 Antibody

Pricing & Availability
Clone
RM3/1 (See other available formats)
Regulatory Status
RUO
Other Names
GHI/61, M130, RM3/1, p155, Hemoglobin/haptoglobin complex receptor, Macrophage-associated antigen, ED2(rat), Macrophage marker
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RM3slash1_PerCpCyanine55_CD163_Antibody_120518
Human peripheral blood monocytes were incubated overnight with recombinant human IL-10, treated with Human TruStain FcX™ (Fc Receptor Blocking Solution) then stained with CD163 (clone RM3/1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • RM3slash1_PerCpCyanine55_CD163_Antibody_120518
    Human peripheral blood monocytes were incubated overnight with recombinant human IL-10, treated with Human TruStain FcX™ (Fc Receptor Blocking Solution) then stained with CD163 (clone RM3/1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
326511 25 tests £86
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326512 100 tests £202
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human monocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone RM3/1 binds to domain 9 of CD163. Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Högger P, et al. 1998. J. Immunol. 161:1883. (FC)
  2. Zwadlo G, et al. 1987. Exp. Cell Biol. 55:295. (FC)
  3. Buechler C, et al. 2000. J. Leukoc. Biol. 67:97. (FC)
  4. Puig-Kroger A, et al. 2009. Cancer Res. 69:9395. (FC) PubMed
  5. Madsen M, et al. 2004. J. Biol. Chem. 279:51561. (FC)
  6. Jones K, et al. 2013. Clin Cancer Res. 19:731. (FC) PubMed
  7. Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
Product Citations
  1. Werz O, et al. 2018. Nat Commun. 9:59. PubMed
RRID
AB_2564016 (BioLegend Cat. No. 326511)
AB_2564017 (BioLegend Cat. No. 326512)

Antigen Details

Structure
134 kD (non-reduced)/155 kD (reduced) glycoprotein, Scavenger receptor superfamily
Distribution

Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells

Function
Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor
Haptoglobin-hemoglobin complex, TWEAK
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Roth J, et al. 1994. Transolantation. 57:127.
2. Van den Heuvel MM, et al.1999. J. Leukoc. Biol. 66:858.
3. Sulahian TH, et al. 2000. Cytokines 12:1312.
4. Fabriek BO, et al. 2007. J. Neuroimmunol. 187:179.

Gene ID
9332 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12/05/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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