PerCP/Cyanine5.5 anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
HCD14_PerCPCyanine55_CD14_Antibody_082218
Human peripheral blood monocytes were stained with CD14 (Clone HCD14) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • HCD14_PerCPCyanine55_CD14_Antibody_082218
    Human peripheral blood monocytes were stained with CD14 (Clone HCD14) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
325621 25 tests £103.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
325622 100 tests £222.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The CD14 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Fang W,et al. 2017. PLoS One. 10.1371/journal.pone.0187088. PubMed
  2. Adhikaree J, et al. 2019. Oncoimmunology. 8:1593803. PubMed
  3. Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed
  4. Singh M, et al. 2019. Nat Commun. 10:3120. PubMed
  5. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  6. Georgouli M et al. 2019. Cell. 176(4):757-774 . PubMed
  7. Washburn ML, et al. 2019. J Immunol. 203:1897. PubMed
  8. Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed
  9. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  10. Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed
  11. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  12. Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed
  13. Genoula M, et al. 2018. Front Immunol. 9:459. PubMed
  14. Antonucci L, et al. 2020. Journal of Immunology Research. 3257.929861. PubMed
  15. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed
  16. van Beek JJP, et al. 2020. Cell Reports. 30(4):1027-1038.e4.. PubMed
  17. Kotturi M, et al. 2011. J Virol. 85:11770. PubMed
  18. Brittan M, et al. 2012. Eur Respir J. 40:206. PubMed
  19. Schilling B, et al. 2013. Int J Cancer. 133:1653. PubMed
  20. Nielsen M, et al. 2015. PLoS One. 10:121516. PubMed
  21. Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed
  22. Bsat M, et al. 2015. J Leukoc Biol. 98: 671 - 681. PubMed
  23. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  24. Ma L, et al. 2021. Clinical Cancer Research. 27(6):1778-1791. PubMed
  25. Jost M, et al. 2021. eLife. 10:00. PubMed
  26. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
RRID
AB_893252 (BioLegend Cat. No. 325621)
AB_893250 (BioLegend Cat. No. 325622)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 08/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account