PerCP/Cyanine5.5 anti-BrdU Antibody

Pricing & Availability
Clone
3D4 (See other available formats)
Regulatory Status
RUO
Other Names
5-bromodeoxyuridine, bromodeoxyuridine
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
3D4_PerCPCY5.5_BrdU_Antibody_ICFC_1_092415
HEL cell line was pulsed with BrdU for one hour (upper panel) or without (lower panel) and then stained with anti-BrdU (clone 3D4) PerCP/Cyanine5.5 according to BioLegend BrdU staining procedure. Cells were subsequently stained with 1 µg of DAPI for DNA analysis.
  • 3D4_PerCPCY5.5_BrdU_Antibody_ICFC_1_092415
    HEL cell line was pulsed with BrdU for one hour (upper panel) or without (lower panel) and then stained with anti-BrdU (clone 3D4) PerCP/Cyanine5.5 according to BioLegend BrdU staining procedure. Cells were subsequently stained with 1 µg of DAPI for DNA analysis.
  • 3D4_PerCPCY5.5_BrdU_Antibody_ICFC_2_092415
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
364109 25 tests £79.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
364110 100 tests £202.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Iodouridine-conjugated ovalbumin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
  2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
  3. Godebu E, et al. 2008. J. Immunol. 181:1798.
  4. Waskow C, et al. 2008. Nat. Immunol. 9:676.
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
RRID
AB_2566453 (BioLegend Cat. No. 364109)
AB_2566454 (BioLegend Cat. No. 364110)

Antigen Details

Structure
Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Distribution

Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.

Function
The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Barker JM, et al. 2013. PLoS One 8:e63692.
2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205.
3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855.
4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954.
5. van der Wath RC, et al. 2009. PLoS One 4:e6972.
6. Dolbeare F, et al. 1985. Cytometry 6:521.
7. Gratzner HG. 1982. Science 218:474.

Gene ID
NA
UniProt
View information about BrdU on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06/01/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-BrdU

  • Alexa Fluor® 488 anti-BrdU

  • FITC anti-BrdU

  • Alexa Fluor® 647 anti-BrdU

  • PerCP/Cyanine5.5 anti-BrdU

  • Phase-Flow™ FITC BrdU Kit

  • Phase-Flow™ Alexa Fluor® 647 BrdU Kit

  • PE anti-BrdU

  • Alexa Fluor® 700 anti-BrdU

  • APC anti-BrdU

  • PE/Cyanine7 anti-BrdU

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account