- B73.1 (See other available formats)
- Regulatory Status
- Other Names
- FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).Product Details
- Antibody Type
- Host Species
- NK cell-enriched fraction from human peripheral blood.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G84.
Donor variability has been observed for clone B73.1 staining1, especially on granulocytes.
(PubMed link indicates BioLegend citation)
- Perussia B, et al. 1983. J. Immunol. 130:2133.
- Lanier LL, et al. 1985. J. Exp. Med. 162:2089.
- Perussia B, et al. 1984. J. Immunol. 133:180.
- Grier JT, et al. 2012. J. Clin. Invest. 122:3769. (Epitope)
AB_2563686 (BioLegend Cat. No. 360719)
AB_2563687 (BioLegend Cat. No. 360720)
- Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells
- Low affinity IgG Fc receptor, phagocytosis, ADCC
- IgG Fc receptor III (FcγRIII)
- Cell Type
- Macrophages, Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M and Martini R. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
5. Björkström NK, et al. 2008. J. Immonol. 181:4219.
- Gene ID
- 2214 View all products for this Gene ID 2215 View all products for this Gene ID
- View information about CD16 on UniProt.org
- How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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