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PE/Dazzle™ 594 anti-mouse CD276 (B7-H3) Antibody

Pricing & Availability
Clone
MIH35 (See other available formats)
Regulatory Status
RUO
Other Names
B7RP2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MIH35_PEDazzle594_CD276_Antibody_092518
Mouse B7-H3-transfected P815 cells were stained with CD276 (clone MIH35) PE/Dazzle™ 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
  • MIH35_PEDazzle594_CD276_Antibody_092518
    Mouse B7-H3-transfected P815 cells were stained with CD276 (clone MIH35) PE/Dazzle™ 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
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135611 25 µg £97
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135612 100 µg £238
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Description

B7-H3 is a type I transmembrane protein belonging to the B7 family of co-stimulatory proteins. B7-H3 is mostly expressed on professional APCs including B cells, macrophages, and dendritic cells at low levels. It is detected on various human and murine tumor cells, nasal and airway epithelial cells. Its expression on dendritic cells appears to be up-regulated by LPS. Initial studies have shown that B7-H3 provides a stimulatory signal to T cells. However, recent studies suggest a negative regulatory role for B7-H3 in T cell responses. Mouse B7-H3 protein inhibited T cell activation and effector cytokine production. Thus, the immunological function of B7-H3 remains unclear. B7-H3 is involved in the suppression of Th1-mediated immune responses and plays an important role in the development of pathogenic Th2 cells in a murine asthma model. Monoclonal antibody against B7-H3 enhances T cell proliferation in vitro and leads to exacerbated EAE in vivo. It has been reported that the Triggering Receptor Expressed on Myeloid cells (TREM)-like Transcript 2 (TLT-2, TREML2) is a receptor for B7-H3 in mice, although it remains controversial. Further studies are needed to identify the receptor of B7-H3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse B7-H3 transfected L cell and P815
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hashiguchi M, et al. 2008. Proc Natl Acad Sci USA. 105(30):10495.
  2. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
RRID
AB_2750519 (BioLegend Cat. No. 135611)
AB_2750520 (BioLegend Cat. No. 135612)

Antigen Details

Structure
A 49.7 kD member of the B7 family of the Ig superfamily proteins.
Distribution

Mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells at low level.

Function
Provides a stimulatory signal to T cells, plays a negative regulatory role in T cell responses as well.
Ligand/Receptor
The receptor has not been completely identified, but it seems to be expressed on activated T cells.
Cell Type
Antigen-presenting cells, B cells, Dendritic cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Nagashima O, et al. 2008. J. Immunol. 181:4062
2. Prasad DVR, et al. 2004. J. Immunol. 173:2500
3. Sun M, et al. 2002. J. Immunol. 168:6294
4. Xu J, et al. 2006. Cellular and Molecular Immunology. 3(3):235
5. Ford JW, et al. 2009. Curr Opin Immunol. 21(1):38
6. Leitner J, et al. 2009. Eur. J. Immunol. 2009. 39(7):1754

Gene ID
102657 View all products for this Gene ID
UniProt
View information about CD276 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/25/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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