PE/Cyanine7 anti-rat CD8a Antibody

Pricing & Availability
Clone
OX-8 (See other available formats)
Other Names
CD8a, T8, Leu-8, Ly-2, Lyt2
Isotype
Mouse IgG1, κ
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Product Citations
publications
OX-8_PE_CYANINE7_CD8a_Antibody_071119
LOU rat splenocytes were stained with CD3 (clone 1F4) APC and CD8a (clone OX-8) PE/Cyanine7 (left) or Mouse IgG1, κ PE/Cy7 isotype control (right).
  • OX-8_PE_CYANINE7_CD8a_Antibody_071119
    LOU rat splenocytes were stained with CD3 (clone 1F4) APC and CD8a (clone OX-8) PE/Cyanine7 (left) or Mouse IgG1, κ PE/Cy7 isotype control (right).
Cat # Size Price Quantity Avail. Save
201715 25 µg £67
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201716 100 µg £158
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Description

CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
High molecular weight glycoproteins from rat thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. The solution is free of unconjugated PE/Cyanine7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The OX-8 antibody has been reported to partially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation. Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation3, in vivo and in vitro blocking of T cell responses3, 4, macrophage stimulation5, and Western blotting3,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Barclay AN. 1981. Immunology 42:593. (IHC)
  2. Wallgren AC, et al. 1995. Transplantation 60:594. (IHC)
  3. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP, WB)
  4. Mason DW, et al. 1983. Immunol. Rev. 74:57.
  5. Hirji N, et al. 1997. J. Immunol. 158:1833. (FA)
  6. Mitnacht R,, et al. 1998. J. Immunol. 160:700. (WB)

Antigen Details

Structure
Ig superfamily, approximately 32 kD
Distribution

Thymocytes, NK cells, most CD8+ cells, intestinal intraepithelial lymphocytes, activated CD4+ cells, macrophages

Function
T cell activation, co-receptor for T cell activation
Ligand/Receptor
MHC class I molecule
Cell Type
Lymphocytes, Macrophages, NK cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Johnson P, et al. 1985. EMBO J. 4:2539.
2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.

Gene ID
24930 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/11/2019

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