- OX-8 (See other available formats)
- Other Names
- CD8a, T8, Leu-8, Ly-2, Lyt2
- Mouse IgG1, κ
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- Product Citations
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CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).Product Details
- Antibody Type
- Host Species
- High molecular weight glycoproteins from rat thymocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. The solution is free of unconjugated PE/Cyanine7 and unconjugated antibody.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The OX-8 antibody has been reported to partially block T cell responses, including mixed lymphocyte reactions and cytotoxic T cell responses, and to induce macrophage activation. Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation3, in vivo and in vitro blocking of T cell responses3, 4, macrophage stimulation5, and Western blotting3,6.
(PubMed link indicates BioLegend citation)
- Barclay AN. 1981. Immunology 42:593. (IHC)
- Wallgren AC, et al. 1995. Transplantation 60:594. (IHC)
- Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP, WB)
- Mason DW, et al. 1983. Immunol. Rev. 74:57.
- Hirji N, et al. 1997. J. Immunol. 158:1833. (FA)
- Mitnacht R,, et al. 1998. J. Immunol. 160:700. (WB)
- Ig superfamily, approximately 32 kD
Thymocytes, NK cells, most CD8+ cells, intestinal intraepithelial lymphocytes, activated CD4+ cells, macrophages
- T cell activation, co-receptor for T cell activation
- MHC class I molecule
- Cell Type
- Lymphocytes, Macrophages, NK cells, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Johnson P, et al. 1985. EMBO J. 4:2539.
2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.
- Gene ID
- 24930 View all products for this Gene ID
- View information about CD8a on UniProt.org
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