PE/Cyanine7 anti-mouse CD272 (BTLA) Antibody

Pricing & Availability
Clone
6A6 (See other available formats)
Other Names
BTLA, B and T lymphocyte attenuator
Isotype
Armenian hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
6A6_PECyanine7_CD272_Antibody_100819
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CD272 (BTLA) (clone 6A6) PE/Cyanine7 (left) or Armenian hamster IgG PE/Cyanine7 isotype control (right).
  • 6A6_PECyanine7_CD272_Antibody_100819
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CD272 (BTLA) (clone 6A6) PE/Cyanine7 (left) or Armenian hamster IgG PE/Cyanine7 isotype control (right).
Cat # Size Price Quantity Avail. Save
139115 25 µg £87
Check Availability


Need larger quantities of this item?
Request Bulk Quote
139116 100 µg £195
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.  BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response.  The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells.  BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo.  Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells.  BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity.  It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm.  The existence of three distinct BTLA alleles has been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6 BTLA Ig domain protein in CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. The solution is free of unconjugated PE/Cyanine7 and unconjugated antibody.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1,2 and blocking3,5 of OT-I T cell responses in vitro and incidence of cerebral malaria in vivo caused by P. berghei ANKA (PbA) infection. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 139104).

Clone 6A6 antibody reacts only with the C57BL/6 allele of BTLA but not the BALB/c allele of BTLA.

Application References

(PubMed link indicates BioLegend citation)
  1. Hurchla MA, et al. 2005. J. Immunol. 174:337. (IP FC)
  2. Sedy JR, et al. 2005. Nat. Immunol. 6:90. (IP)
  3. Lepenies B, et al. 2007. J. Immunol. 179:4093. (Block)
  4. Tao R, et al. 2005. J. Immunol. 175:5774. (FC)
  5. Albring J, et al. 2010. J. Exp. Med. 207:2551. (Block)

Antigen Details

Structure
An Ig superfamily co-inhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
Distribution

BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+ T cells, lower expression on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.

Function
BTLA functions as a negative regulator of T cell activation and proliferation, attenuate B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
Ligand/Receptor
HVEM.
Cell Type
B cells, Dendritic cells, Lymphocytes, NKT cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Liu X, et al. 2009. J. Immunol. 182:4516.
2. Miller ML, et al. 2009. J. Immunol. 183:32.
3. Sun Y, et al. 2009. J. Immunol. 183:1946.
4. Vendel AC, et al. 2009. J. Immunol. 182:1509.
5. Watanabe N, et al. 2003. Nat. Immunol. 4:670.

Gene ID
208154 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/08/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account